姜黄素衍生物c085抗慢性粒细胞白血病的研究

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1、分类号：R96学校代码：10392学科专业代码：100706学号：200703081福建医科大学硕士学位论文姜黄素衍生物C085抗慢性粒细胞白血病的研究EffectofCurcuminDerivativeC085onChronicMyelogenousLeukemiaCells学位类型：医学硕士所在学院：药学院申请人：吴莺学科、专业：药理学导师：许建华教授研究起止日期：2009年1月至2013年3月答辩日期：2013年5月30日二○一三年五月姜黄素衍生物C085抗慢性粒细胞白血病的研究目录中文摘要·························

2、···································································1英文摘要····························································································3英文缩略词表······················································································5前言················

3、············································································6第一部分姜黄素衍生物C085体外抗慢性粒细胞白血病作用1实验材料与仪器···············································································81.1细胞系··············································································

4、···········81.2主要药物和试剂·············································································81.3主要仪器······················································································81.4主要溶液配制·········································································

5、·······92实验方法·························································································92.1C085对细胞增殖的影响·································································92.1.1细胞培养···················································································

6、·92.1.2MTT法观察对细胞增殖的影响·······················································92.1.3台盼蓝排染法观察对细胞增殖的影响··············································102.2AnnexinV--FITC/PI双荧光染色法检测细胞凋亡································10+2.3CML病人白血病骨髓CD34细胞分离与培养····································10+

7、2.4集落形成法观察对CML病人白血病骨髓CD34细胞增殖的影响···········112.5统计学方法··············································································113结果·····························································································113.1C085对CML细胞株增殖的影响··························

8、························113.1.1C085对K562细胞增殖的抑制作用···················