慢性肾功能不全通过下调abca1表达影响巨噬细胞内胆固醇含量

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1、慢性肾功能不全通过下调ABCA1表达影响巨噬细胞内胆固醇含量周加军1，甘华1，刘彩欣2，陈力学3，汤为学3（400016重庆，重庆医科大学第一附属医院：肾脏科1，神经内科实验室3；266500青岛，青岛市经济技术开发区第二人民医院检验科2）[摘要]目的探讨慢性肾功能不全患者巨噬细胞内胆固醇含量的变化及ABCA1的表达情况。方法选取2010年8月至2012年3月本院住院的慢性肾功能不全1.2中写的是“慢性肾衰患者”，请全文统一写法，体现撰文的严谨性（chronicrenalfailure，CRF）患者30例和健康对照组健康者？病人？交待不清20例。分离外周血单核细胞诱导贴壁巨噬化，

2、油红O染色鉴定,应用酶法测定巨噬细胞内胆固醇含量，应用real-timePCR与Westernblot方法测定细胞ATP结合盒受体A1(ATPbindingcassettetransporter-A1，ABCA1)的mRNA及蛋白表达。结果慢性肾功能不全患者巨噬细胞内胆固醇含量明显高于健康对照组，ABCA1在慢性肾功能不全患者巨噬细胞中的mRNA与蛋白表达显著低于健康对照组全文请统一写法。结论慢性肾功能不全患者巨噬细胞的胆固醇含量明显增加，胆固醇含量增加与慢性肾功能不全患者ABCA1的表达下调有关。[关键词]ABCA1；胆固醇含量；慢性肾功能不全[中图法分类号][文献标志码]A[

3、通信作者]甘华，E-mail：zxg0233@sina.com[基金项目]重庆市卫生局重点项目（2010-1-16）ChronicRenalFailureInfluencesMacrophagesCholesterolcontentbyRepressingABCA1ZhouJiajun1,GanHua1,LiuCaixin2,ChenLixue3,TangWeixue3（1DepartmentofNephrology,3Neurologydepartmentlaboratory，FirstAffiliatedHospitalofChongQingMedicalUniversity

4、,ChongQing400016；2ClinicalLaboratory，TheSecondPeople'sHospitalofQingdaoeconomicandtechnicaldevelopmentzone，QingDao266500）[Abstract]Objective:toexplorethechangesofcholesterolcontentandtheexpressionsofABCA1inchronicrenalfailure(CRF)patients.Methods:BetweenAug.2010andMar.2012,weinvestigated30CRF

5、patients,and20healthyvolunteers.Peripheralbloodmononuclearcells(PBMCs)wereisolatedandstimulatedtomakethemtransfertomacrophages.MacrophageswereidentifiedbyOilredOdyeing.Thecellularcholesterolcontentswasdetectedbyenzymaticcolorimetry.MacrophageswerecollectedtodetecttheexpressionofABCA1byusingRe

6、al-timePCRandWesternblot.Results:Comparedtothenormalcontrol（CON）,thecontentofintracellularcholesterolinCRFpatientswassignificantlyhigherthanthatinhealthypatients,theexpressionofABCA1wassignificantlydecreasedinCRF（p<0.01）.Conclusion：CRFincreasecholesterolcontentinhumanmonocyte-macrophagederive

7、dfoamcells,themechanismofwhichmaybeassociatedwithincreaseofexpressionofABCA1.[Keywords]ABCA1；cholesterolcontent;chronicrenalfailureSupportedbytheKeyProjectofChongqingMunicipalHealthBureau(2010-1-16).Correspondingauthor：GanHua,E-mail:zxg0233@s