neurod对短暂缺氧性脑损伤模型小鼠的学习记忆功能及其脑内神经干细胞分化作用的影响

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1、分类号：R741.05学校代码：10392学科专业代码：100204学号：2101003343福建医科大学硕士研究生毕业论文NeuroD对短暂缺氧性脑损伤模型小鼠的学习记忆功能及其脑内神经干细胞分化作用的影响InfluenceofNeuroDtothefunctionoflearningandmemoryaswellthedifferentiationofintracerebralneuralstemcellinmousemodeloftransienthypoxia学位类型：医学硕士所在学院：省立临床医学院研究生：杜海燕学科、专业：神经病学导师：季晓林教授研究起

2、止日期：2011年10月至2013年1月答辩日期：2013年5月30日二○一三年五月1目录英文缩略词表·······················································1中文摘要···························································2英文摘要···························································3前言················································

3、·············5第一部分短暂缺氧性脑损伤新生小鼠模型的建立与鉴定1引言·······················································72材料与方法···················································73结果·······················································124讨论·······················································175小结·······

4、················································20第二部分NeuroD对短暂缺氧性脑损伤模型小鼠的干预效果观察1引言·······················································212材料与方法···················································213结果·······················································294讨论·······················

5、································325小结·······················································35结论···························································35参考文献··························································36致谢···························································38综

6、述···························································39英文缩略词表(Abbreviation)缩写英文全称中文全称NeuroDneurogenicdifferentiation神经源性分化因子bHLHbasichelix-loop-helix碱性螺旋-环-螺旋PBSPhosphatebuffersaline磷酸盐缓冲液BrdU5-bromo-2-deoxyuridine5-溴-2-脱氧尿嘧啶cDNAcomplementarydeoxyribonucleic互补脱氧核糖核酸acidDEPCdiet

7、hylpyrocarbonate焦磷酸二乙酯ddH2Odoubledistillationwater双蒸水EBethidiumbromide溴化乙锭EPeppendorf微量离心管FITCfluoresceinisothiocyanate异硫氰酸荧光素mRNAmessengerribonucleicacid信使核糖核酸RT-PCRreversetranscriptionpolymerase逆转录聚合酶链式反应chainreactionSPSSstatisticalpackageforsocial社会科学统计程序scienceTBETris-Boricacid-ED

8、TA三羟甲