资源描述:
《海杧果繁殖及混交技术的研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、及诱导芽发生叶片最佳消毒处理是0.1%升汞消毒7min,用0.1%升汞连续两次消毒(7min+5min)是海杧果茎段消毒的最适宜时间;活性炭和VC都可以降低海杧果叶片的褐变率和褐变程度,活性炭的浓度为1g/L时的褐变率最低(37.8%),而VC浓度在1.5g/L时,褐变率最低(48.9%),两者比较,前者的防褐变效果更佳。诱导叶片产生愈伤组织的最适合培养基是MS+2,4-D5mg/L,并在黑暗条件下培养,诱导率达40.91%;促使茎段膨大效果最好的培养基是MS+2mg/L2,4-D+0.5mg/L6-BA,诱导茎段芽发生最快的培养基组合是M
2、S+2mg/L6-BA+0.4mg/LNAA+0.5mg/LGA3。(4)海杧果混交效果3种混交林分中,木麻黄成过熟林下的海杧果幼苗存活率最高,达88.9%,其存活率显著高于木麻黄幼龄林及中龄林,存活率最低的为木麻黄幼龄林;三个处理中,添加凋落物处理的海杧果生长情况最佳;三种林分中,光合能力大小的顺序为成熟林>中龄林>幼-2龄林;叶绿素含量表现为成熟林最高为47.62mg.m,最低的是幼龄林,叶绿素含量为-233.98mg.m;3种木麻黄林分中,成过熟林郁闭度最大,光照度最弱,但海杧果成活率较高,长势较好表明海杧果幼年阶段具有喜阴的生物学特
3、性。综上所述,海杧果与木麻黄混交具有造林成活率高,种子萌发率高,生长性能良好,且有裸苗易成活的特点。此外,海杧果具有天然更新能力强,耐盐碱、耐贫瘠、抗风等优点,是沙质海岸木麻黄海防林混交最佳的伴生树种。但海杧果天然资源分布范围有限,数量小,受人为干扰破坏严重,建议有关部门能对海杧果种群进行保护。关键词:海南岛;海杧果;种质资源调查;种子萌发;组织培养;混交种植IV万方数据AbstractCerberaManghasisoneofthenativespeciesinHainanIsland,growingoncoastalzone.Ithas
4、characteristicsofwindresistance,toleranceofsoildepletionandsaline-alkalinesoil,strongnaturalregenerationability,etc.ThisprojectmainlyinvolvedintheinvestigationofCerberaManghasgermplasmresource,itsbreedingcharacteristics,andmixed-speciesplantationtechnologytoprovidetheoreti
5、calbasisfortheselection,utilizationanddevelopmentofalternativetreespeciesforCasuarinaequisetifoliashelterbeltsinHainanIsland.BasedontheGoogleEarthmap,theinvestigationzonesofCerberaManghagermplasmresourcewasdetermined.CerberaManghasgermplasmresourceoffivecitiesandcountiesin
6、NorthernHainanIsland,includingHaikou,Wenchang,Qionghai,DengmaiandLingao,wereinvestigatedwiththecombinationofon-sitesurveyandtypicalsampleplots,inordertounderstandgeneraldistributionsandpopulationcharacteristicsofCerberaManghas7germplasmresourceinthezonesmentionedabove.L8(2
7、)thogonalexperimentdesignforthegerminationofCerberaManghasseedswasadoptedtoanalyzetheinfluencesofgrowthhormone(A),hormoneconcentration(B),soilmatrix(C)andlightconditions(D)onseedgerminationandseedling/saplinggrowthofCerberaManghas.Intissueculture,explantsofCerberaManghaswe
8、retakenfromstemswithaxillarybudsandtenderleaves;leafcallusandstemgerminationswereinducedb