三叶木通组织培养与多倍体诱导

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1、贵州大学硕士研究生论文三叶木通组织培养及多倍体诱导TissueCultureandPolyploidInductioninAkebiatrifoliateSummaryInourresearchwildAkebiatrifoliatewasusedasexperimentalmaterial.BytissuecultureweestablishedrapidpropagationofAkebiatrifoliate.Also,colchicinewasusedforpolyploidinductionofcluste

2、rbuds.Thenpolyploididentificationwascarriedout.TheselaidthefoundationofrapidpropagationofAkebiatrifoliate.Andprovidedtechnicalsupportfornewvarietiescultivation.Themainresultsareasfollows:1.Selectionandtreatmentofexplant.Seedsremovedfromfruitwerethebestexplantin

3、thestudyofcombinativesterilizationofalcoholandmercuricchloridetodifferentexplants.75%alcoholdisinfect15secondsandthen0.1%mercuricchloridesterilize7minuteswasanoptimalscheme.TheoptimumconditionofseedlingemergenceofAkebiatrifoliatewascuttingseedsinhalflengthwisea

4、ndinoculatingintoMS+2Ca2++6-BA1.0mg/L+NAA0.3mg/L+activatedcarbon0.1g/Lmediumafterthat.Theinoculatedseedswerecultivatedontheconditionoflighting12hoursperdayunder25℃with1000lxlightintensity.2.Inductionanddifferentiationofseedcallus.Theoptimizedinducingmediumforse

5、edcalluswasMS+2,4-D1.0mg/L+6-BA0.3mg/Lmedium.Onthisconditiontheinductionratewas32.5%.EmbryogeniccalluscanbeobtainedafterseedcallussubculturedonMS+2,4-D1.5mg/L+6-BA0.1mg/Lmedium.EmbryogeniccallusdifferentiationoccurredonMS+6-BA2.0mg/L+NAA0.3mg/L+75g/Lbananapuree

6、medium.Differentiationrateofembryogeniccalluswas11.7%.3.Inductionandrootingcultureofclusterbuds.ThesuitablemediumforaxillarybudsinductionwasMS+6-BA2.0mg/Lmedium.Stemwithaxillarybudscanbeinducedtogrowaveragebudsnumberupto8budsonMS+6-BA2.0mg/L+2,4-D0.2mg/Lmedium,

7、however,weonlygotmainbudandotherbudswhichcouldnotdifferentiationintoaxillarybuds.Clusterbudsinducedbysingleaxillarybudalsofailedinthisstudy.OntheMS+6-BA2.0mg/L+NAA0.3mg/Lmediumseedlingaverageproliferationnumberwasthehighestandthenthismedium2万方数据贵州大学硕士研究生论文三叶木通组

8、织培养及多倍体诱导wasselectedastheclusterbudsinductionmediumofseedling.Singleaxillarybudcannotrootunderdifferenthormonecombinations,whilemultiplebudclumpscanrootunderdifferenthormone