放射线对balb2fc小鼠畸变产物耳声发射变化和耳蜗外毛细胞prestin表达影响的实验观察

《放射线对balb2fc小鼠畸变产物耳声发射变化和耳蜗外毛细胞prestin表达影响的实验观察》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、硕士学位论文ExperimentalObservationofInfluenceofRadiationonDistortionProductOtoacousticEmissionandthePrestinProteinExpressionintheMiceOuterHairCellsobjective：Name：ZhaoXiangdongSupervisor：Pro￡LiangYongABSTRACT1。Tostudytheeffectofsingle-doseofradiationondistortionproductotoacousticemission(DPOAE)andthePres

2、tinproteinexpressionincochlearouterhairceilsofmice．2．Tostudytheeffectofdifferentdosesofradiationondistortionproductotoacousticemission(DPOAE)andthePrestinproteinexpressionincochlearouterhaircellsofmice．3．Toexplorethepossiblemechanismofradiotherapycausingsensorineuralhearingloss(SNHL)．Materials＆Meth

3、ods：1．MaterialsFourweeksoldmaleBalb／cmice，whichwerenormal，excludedtheinfectioninexternalauditorycanalsandmiddleears，weighing17-229，theaverageWaSabout209，purchasedfromtheAnimalCenterofSouthernMedicalUniversity,wereIABSTRACTrandomlydividedintoexperimentalgroupsandblankcontrolgroup．2．Methods：2．1Establ

4、ishmentofmicemodelwithSNHLafterradiotherapyAllthemicewereanaesthetized研廿12％sodiumpentobarbital(0．3ml／100g)afterweighted．Theywerekeptinthesupineposition,andWedefinedtheinnerearradiotherapyrangebyX-rayorientation．Theexperimentalmicewereirradiatedontherightears、耐tllstateddosesof6MeVelectronsfromalinea

5、raccelerator(Varian2100，America)．Miceweretreated谢tllthesourceskindistanceof100cm,doserateof400cGy／minandsubcutaneousreferencedepthof1．Scm．Allthemiceundergoingradiotherapywereprovidedwithsheetleadstoprotectthedigestivecanalandrespiratorytract．ThenwetestedvariousindexsignofDPOAEandtheprestinproteinex

6、pressionincochlearouterhaircellsofmice．2．2Invivoacousticemissionassessments(DPOAE)Allthemicewereanaesthetizedwith2％sodiumpentobarbital(0．3ml／100g)andtheresponseamplitudeandsigrlaltonoiseratioofdistortionproductotoacousticcmission(DPOAE)wererecorded．Acubicdistortionproductof2fl-f2wasrecordedfromfreq

7、uency0．5-12kHzwitllf2／fl=1．22atintensityofL1／L2=80／80dBSPL．DPOAEWaSmeasuredbeforeandafterexposuredtotheradiation．2．3PresfinWesternblottingThewholecochleawascrushedinamodifiedRIPAcell1ysisbuffer(i0mivlTris，1