鲑鱼降钙素对去卵巢大鼠骨代谢与骨组织rumx22fpparγ和opg2frankl表达影响的实验分析

《鲑鱼降钙素对去卵巢大鼠骨代谢与骨组织rumx22fpparγ和opg2frankl表达影响的实验分析》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、达下降，但骨髓腔脂肪细胞及PPARvmRNA表达无明显下降。降钙素可以增加胫骨干骺端OPG蛋白和股骨骨髓细胞OPGmRNA表达，但是对RANKL蛋白和mRNA表达无明显减少。【结论】1．去卵巢后大鼠骨组织RANKL及PPARy表达增强，分别促进破骨细胞和脂肪分化。并且RUNX2反应性增强，促进成骨细胞分化。因此，大鼠去卵巢后最终表现出骨量丢失和高转换骨代谢。2．sCT能预防去卵巢大鼠骨量丢失和抑制骨转换。可能的机制是sCT增强OPG表达通过OPG／RANKL／RANK系统抑制破骨细胞分化。并且RUNX2表达反应性下调，抑制了成骨细胞分化。【关键词】去卵巢大鼠；

2、转录因子；核因子KB受体活化因子配体；骨保护素；骨髓细胞；降钙素2EffectofSalmonCalcitoninonbonemetabolismandRUNX2／PPARyandOPG／RANKLexpressioninbonetissuesinOvariectomizedRats【ObjectivelAbstract1．Toexplorethechangesinbonemineraldensity,bonemetabolismandrelatedgeneandproteinexpressinforosteoblastogenesis，adipogenesi

3、sandosteoclastogenesisinbonetissuesinovariectomizedrats．2．Toexploretheeffectofsalmoncalcitonin(sCT)onbonemineraldensityandbonemetaboblisminovariectomizedrats．3．Toexploretheeffectofsalmoncalcitoninonrelatedgeneandproteinexpressinforosteoblastogenesis，adipogenesisandosteoclastogenesis

4、inbonetissuesinovariectomizedrats．【Methods】TwentyfourhealthyfemaleSDrats，3monthsold，wererandomlydividedintothreegroups(n=8each)：shamgroup，OVXgroupandsCTgroup．Theosteoporoticratmodelwasestablishedbybilateralovariectomy．ThesCTgroupreceivedsalmoncalcitoninatadoseof2IU／kg／dS．C．whiletheo

5、thertworeceivedO．9％salineatadoseof5ml／kg／dS．C．12weeksaftersCTadministration，thebonemineraldensity(BMD)offemurandthefourthlumbarvertebrawasmeasuredbyDXA．TheconcentrationsofPICP,BGPandITCPinratserumweremeasuredbyELISA．ThebonetrabeculaewereobservedbyHEstaininginproximaltibiaandthethird

6、lumbarvertebra．ThenumberofadipocyteswerecountedbyHEstaininginproximaltibiaandthethirdlumbarvertebra．TheproteinexpressionforOPGandRANKLinbonetissuesofproximaltibiawasmeasuredbyimmunohistochemical．QRT-PCRwasusedformRNA1evelsofRUNX2，PPARY，OPGandRANKLinbonemarrowcells．【Results】1．Compare

7、dwiththeshamgroup，BMDwasreducedsignificantlyinthe3lumbarvertebraeandfemursfromovariectomizedrats．BonetumovermarkersBGP,PICP,andICTPwereincreasedsignificantlyinovariectomizedratserum．Comparedwiththeshamgroup，thegeneexpressionforRUNX2andPPARYinbonemarrowcellsandthenumberofadipocytesin

8、bonemarrowcavitywer