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ID:33913178
大小:7.93 MB
页数:40页
时间:2019-03-02
《鲑鱼降钙素对去卵巢大鼠骨代谢与骨组织rumx22fpparγ和opg2frankl表达影响的实验分析》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、达下降,但骨髓腔脂肪细胞及PPARvmRNA表达无明显下降。降钙素可以增加胫骨干骺端OPG蛋白和股骨骨髓细胞OPGmRNA表达,但是对RANKL蛋白和mRNA表达无明显减少。【结论】1.去卵巢后大鼠骨组织RANKL及PPARy表达增强,分别促进破骨细胞和脂肪分化。并且RUNX2反应性增强,促进成骨细胞分化。因此,大鼠去卵巢后最终表现出骨量丢失和高转换骨代谢。2.sCT能预防去卵巢大鼠骨量丢失和抑制骨转换。可能的机制是sCT增强OPG表达通过OPG/RANKL/RANK系统抑制破骨细胞分化。并且RUNX2表达反应性下调,抑制了成骨细胞分化。【关键词】去卵巢大鼠;
2、转录因子;核因子KB受体活化因子配体;骨保护素;骨髓细胞;降钙素2EffectofSalmonCalcitoninonbonemetabolismandRUNX2/PPARyandOPG/RANKLexpressioninbonetissuesinOvariectomizedRats【ObjectivelAbstract1.Toexplorethechangesinbonemineraldensity,bonemetabolismandrelatedgeneandproteinexpressinforosteoblastogenesis,adipogenesi
3、sandosteoclastogenesisinbonetissuesinovariectomizedrats.2.Toexploretheeffectofsalmoncalcitonin(sCT)onbonemineraldensityandbonemetaboblisminovariectomizedrats.3.Toexploretheeffectofsalmoncalcitoninonrelatedgeneandproteinexpressinforosteoblastogenesis,adipogenesisandosteoclastogenesis
4、inbonetissuesinovariectomizedrats.【Methods】TwentyfourhealthyfemaleSDrats,3monthsold,wererandomlydividedintothreegroups(n=8each):shamgroup,OVXgroupandsCTgroup.Theosteoporoticratmodelwasestablishedbybilateralovariectomy.ThesCTgroupreceivedsalmoncalcitoninatadoseof2IU/kg/dS.C.whiletheo
5、thertworeceivedO.9%salineatadoseof5ml/kg/dS.C.12weeksaftersCTadministration,thebonemineraldensity(BMD)offemurandthefourthlumbarvertebrawasmeasuredbyDXA.TheconcentrationsofPICP,BGPandITCPinratserumweremeasuredbyELISA.ThebonetrabeculaewereobservedbyHEstaininginproximaltibiaandthethird
6、lumbarvertebra.ThenumberofadipocyteswerecountedbyHEstaininginproximaltibiaandthethirdlumbarvertebra.TheproteinexpressionforOPGandRANKLinbonetissuesofproximaltibiawasmeasuredbyimmunohistochemical.QRT-PCRwasusedformRNA1evelsofRUNX2,PPARY,OPGandRANKLinbonemarrowcells.【Results】1.Compare
7、dwiththeshamgroup,BMDwasreducedsignificantlyinthe3lumbarvertebraeandfemursfromovariectomizedrats.BonetumovermarkersBGP,PICP,andICTPwereincreasedsignificantlyinovariectomizedratserum.Comparedwiththeshamgroup,thegeneexpressionforRUNX2andPPARYinbonemarrowcellsandthenumberofadipocytesin
8、bonemarrowcavitywer
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