活血开窍汤对大鼠脑缺血再灌注损伤no含量与细胞凋亡的影响

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1、天津医科人学硕士学位论文KaiQiaoHuoXue．decoction，adultmaleWistarratswereusedasexperimentalanimals．MaleWistarratswererandomlydividedinto5groups：blankgroup，shamgroup，modelgroupandtherapygroup．Everygrouparebreedindifferentcagesunderthesamecondition．Ratsofthetherapygroupwereintragastricallyadministeredtheapprop

2、riatedosesofChinesemedicineHuoXueKaiQiao-decoction，andthesamedosesofPhysiologicalSalinetotheshamandmodelgroupseachdayfor5daysbeforeischemiainduction，andadministrationwascontinueduntilsacrificeatconclusionoftheexperiment．Thecerebralischemiawasinducedby90minofmiddlecerebralarteryocclusion(MCAO)，w

3、hichwasbasedonthemethoddescribedinitiallybyZea．Longa[1Slwithimprovement．At24hoursand48hoursaftel‘themodelsweremade，werapidlykilltheratsandcompletelytakeoutofthebraintissue，halfofwhicharemadetohomogenatetodetecttheNOcontent．Theotherbrainaretakenafterheartperfusionbyparaformaldehyde，andtobeusedto

4、observetheApoptosisandpathologicalchangebythewayofstaining．WedetectthecontentofNOinthebraintissuefluidsamplesbyusingnitratereductasemethodinstrictaccordancewiththestepsofthekit，observetheapoptosisbyTUNEL，andpathologicalchangebyhematoxylinandeosinstain．ResuItsByexperimentalobservation，pathologic

5、alobservationsindicatethatafter24h，48hthenumberofthedamagednervecelloftheratsthattreatedbyHuoXueKaiQiao．decoctionwaslarge，anddamagedseverely．Whilethedegreeofdamagearesignificantlylighterinthetherapygroup．ItwasdeterminedthatthecontentofNOandtheneuronalapoptosisinthebraintissueofratsinmodelgroupw

6、eresignificantlyincreased(P

7、and48hoursandmitigatethedamagetothebrainIV大津医科人学硕士学位论文tissuebyreducingthecontentofNO，theexperimentalresultsalsoshowthattheHuoXueKaiQiao-decoctioncaninhibitthedevelopmentofischemiccellsapoptosis，delayorpreventtheneuronaldeathproces