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ID:33867741
大小:2.38 MB
页数:42页
时间:2019-03-01
《活血开窍汤对大鼠脑缺血再灌注损伤no含量与细胞凋亡的影响》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、天津医科人学硕士学位论文KaiQiaoHuoXue.decoction,adultmaleWistarratswereusedasexperimentalanimals.MaleWistarratswererandomlydividedinto5groups:blankgroup,shamgroup,modelgroupandtherapygroup.Everygrouparebreedindifferentcagesunderthesamecondition.Ratsofthetherapygroupwereintragastricallyadministeredtheapprop
2、riatedosesofChinesemedicineHuoXueKaiQiao-decoction,andthesamedosesofPhysiologicalSalinetotheshamandmodelgroupseachdayfor5daysbeforeischemiainduction,andadministrationwascontinueduntilsacrificeatconclusionoftheexperiment.Thecerebralischemiawasinducedby90minofmiddlecerebralarteryocclusion(MCAO),w
3、hichwasbasedonthemethoddescribedinitiallybyZea.Longa[1Slwithimprovement.At24hoursand48hoursaftel‘themodelsweremade,werapidlykilltheratsandcompletelytakeoutofthebraintissue,halfofwhicharemadetohomogenatetodetecttheNOcontent.Theotherbrainaretakenafterheartperfusionbyparaformaldehyde,andtobeusedto
4、observetheApoptosisandpathologicalchangebythewayofstaining.WedetectthecontentofNOinthebraintissuefluidsamplesbyusingnitratereductasemethodinstrictaccordancewiththestepsofthekit,observetheapoptosisbyTUNEL,andpathologicalchangebyhematoxylinandeosinstain.ResuItsByexperimentalobservation,pathologic
5、alobservationsindicatethatafter24h,48hthenumberofthedamagednervecelloftheratsthattreatedbyHuoXueKaiQiao.decoctionwaslarge,anddamagedseverely.Whilethedegreeofdamagearesignificantlylighterinthetherapygroup.ItwasdeterminedthatthecontentofNOandtheneuronalapoptosisinthebraintissueofratsinmodelgroupw
6、eresignificantlyincreased(P7、and48hoursandmitigatethedamagetothebrainIV大津医科人学硕士学位论文tissuebyreducingthecontentofNO,theexperimentalresultsalsoshowthattheHuoXueKaiQiao-decoctioncaninhibitthedevelopmentofischemiccellsapoptosis,delayorpreventtheneuronaldeathproces
7、and48hoursandmitigatethedamagetothebrainIV大津医科人学硕士学位论文tissuebyreducingthecontentofNO,theexperimentalresultsalsoshowthattheHuoXueKaiQiao-decoctioncaninhibitthedevelopmentofischemiccellsapoptosis,delayorpreventtheneuronaldeathproces
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