欢迎来到天天文库
浏览记录
ID:33865046
大小:4.28 MB
页数:35页
时间:2019-03-01
《黄芪甲苷拮抗镉致心肌细胞损伤的作用》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、重庆医科大学硕士研究生学位论文高倍镜下部分细胞可见胞内呈细丝状,交错排列的肌丝及电子密度较高的Z线样物质。24h的MTT细胞存活率实验结果显示,镉组较对照组心肌细胞的存活率明显降低;镉加不同浓度黄芪甲苷组与对照组比较,存活率虽有明显的降低,但明显高于镉组,且镉加黄芪甲苷(20mg·L’1)组存活率是最高的。透射电镜观察到镉组心肌细胞核呈现不规则分叶,甚至裂解形成高电子密度碎块,胞质固缩,内质网腔明显扩张,线粒体肿胀并出现空泡化及髓鞘样结构,极个别细胞呈现典型凋亡形态;而镉加黄芪甲苷组的心肌细胞超微结构损伤均较
2、镉组轻。②体内实验:与正常组相比,镉组光镜和透射电镜下心肌细胞和闰盘超微结构破坏严重,免疫组织化学结果显示连接蛋白43的表达量明显减少、分布紊乱,实时荧光定量PCR结果显示连接蛋白43mRNA的表达量亦显著降低;而镉加黄芪甲苷组心肌细胞和闰盘的超微结构损伤明显减轻,连接蛋白43的表达量较镉组显著增加。结论:镉能影响原代培养心肌细胞存活率和超微结构、并破坏在体大鼠心肌细胞及闰盘的超微结构、影响连接蛋白43的表达分布及其转录;黄芪甲苷能拮抗镉对心肌细胞的毒性损伤。关键词:镉,黄芪甲苷,心肌细胞,闰盘,连接蛋白43
3、重庆医科大学硕士研究生学位论文ASTRAGALOSIDEANTAGoNIZESTHEDAMAGEEFFECTSOFCADMIUMONCARDIOMYOCYTEINRATABSTRACTobjective:Toinvestigatetheeffectofcadmium(Cd)onthecellviability,intercalateddiscultrastructure,expressionofConnexin43(Cx43)andlevelsofCx43mRNAinratcardiomyocytes,and
4、theprotectionmechanismofastragalosideIV(AsIV)onit.Method:@Theprimaryculturecardiomyocyteswereobtainedfrom1-3daysoldSDneonatalratventriculartissue,andwereidentifiedbyinvertmicroscope,electronmicroscopeobservation,H—Estainingandimmunofluorescence.Then,theprim
5、arycultruecardiomyocytestreatedwithCdorCd+AsIVwereusedforMTTandelectronmicroscopeobservation.②21SDratswererandomlydividedinto3group:controlgroup,Cdgroup(ratsweretreatedwithCd1mg·dq.kg。1bodyweightbyintraperitonealinjection),Cd+AsIVgroup(thedoseofcadmiumwas1m
6、g·dLkg~bodyweight,thedoseofAslVwererespectively5mg·d-1·k’1bodyweightand10mg·d.1·kg~bodyweight).Theratsaftertreatment1weekor2weekswereanesthetizedforthoracotomy,theventriculartissuewererespectivelyadoptedforlight,electronmicroscopeobservation,immunohistochem
7、istryandRT-qPCR.4重庆医科大学硕士研究生学位论文Results:@Theprimaryculturecardiomyocyteswereirregularfusiformorpolygonbylightmicroscopeobservation.Theelectronmicroscopeobservationshowedthatoblongcardiomyocytes,obviousnucleus,abundantmitochondria,Golgibodiesandendoplasmicre
8、ticulum.Athighmagnification,staggeredmyofilamentandZlinematerialwerefoundincardiomyocytes.24hMTTassayshowedthatThecellviabilitywassignificantlyreducedinCdgroupcomparedwithcontrolgroup,anditwassignifica
此文档下载收益归作者所有