波尔定对胶原诱导性关节炎大鼠的治疗作用与对继发性骨质疏松骨微结构的影响

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1、2．波尔定可以通过上调IL．10水平和下调IL。17的水平，抑制异常的自身免疫反应，改善关节滑膜炎性病变，这可能是其发挥作用的机制之一。关键词：波尔定，类风湿关节炎，胶原诱导性关节炎，骨质疏松Ef扎ctsofBoldine1陌eatmentofOsteoporosisBoneMicrostructureinCollagenInducedArthritisRatsABSTRACTObjectiVe：Todetecte髓ctsofb01dineinthecollageninducedanhritis(CIA)ratmodelandthepossiblemechanis

2、mofaction．Methods：TheCIAratmodelwasestablishedinanrats，exceptthoseintheblankcontrolgroupusingboVinetypeIIcollagenaJldincompleteFreund、sa由uVant．ThentheratsweresubdiVidedintothef01lowinggroups：(1)blankcontrolgroup；(2)CIAmodel；(3)MTXgroup(3mg瓜g，twice／week)；(4)thelowdoseofb01dinegroup(2mg

3、／l(∥d)；(5)thehighdoseofboldinegroup(4mg／l(∥d)．DmgtreatmentwasgiVenorallybegiIⅡ1ing15daysaRerthefirstimmunizationfor28days．Dumgtheperiodofthedrugtreamlent，thegeneralsituationofratswasobseⅣed，ratbodyweightmeasured，AIchangesjudgedandanHeswellingdegreemeasuredwiththeVemiercaliper．ARerthee

4、ndoftreatment，theexpression1eVelsoftheinflammato巧cytokinesinserumbyeI磁ymelinkedimmunosorbentmethodsuchasIL一1OwasperfomedinCIArats．5Meanwhile，HEstainingwasexecutedtoobserVethehistopath0109ychangesoftheanⅪeandimmunohistochemicalstainingmethodcarriedouttodetecttheexpI．essionleVelofIL一1Oa

5、ndIL一17inlocalratanl(1ejoint．Aswellasindicesofthymusandspleenwasobse]rVed．AllofthesewerecompletedtoeValuatetheefjfectofb01dineandexploretheirmechanism．Thentakethevertebrae(1umbar)andfemurforthedetectiononBMDandmicro—CT．Results：lEValuationan¨tisindeXandgeneralstate：Comparedwithblankcon

6、trolgroup，ratsofmodelgrouphadsignificantlyweightloss，actiVitiesreducedandspiritwaspoor．ProgressionofarthritissyIllptomaRerbuildingespeciallyasthesymmetrichind1imbsjoimsignificantly．Thepeakofanntisperf．oHIlanceappearsin4weeksaRertheimmune，thengraduallyintothechronicphasewithjointstifme

7、ssanddefomity．2Anl(1ejointswellingandhistopath0109icalresultsshowed：thehighdoseofboldinegroupcouldsigni6cantlyaUeViatethedegreeofinflammationinmodelrats．Duringtreatment，onday1O，AIValuesofhighdoseofboldinegroupwaslowerthanthatoftheCIAgroup(尸

8、feach