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1、猪骨蛋白对骨质疏松大鼠血中AKP和OST的影响第20卷2011掘第1期3月河南医学研究HENANMEDICALRESEARCHVol_2ONo.1March201l文章编号:1004-437X(2011)01-0004-03猪骨蛋白对骨质疏松大鼠血中AKP和OST的影响安玉会,杨丽君,沈永杰,宋丹,贺司宇(郑州大学基础医学院生物化学与分子生物学教研室河南郑州450052)摘要:ii的:探讨猪骨蛋白对骨质疏松大鼠血中碱性磷酸酶(AKP)和骨钙素(OST)水平的影响.方法:取3月龄Wistar大鼠6O只,随机分成对照组(A组),生理盐水组(
2、B组),阳性对照组(C组),200mg骨蛋白组(D组),100mg骨蛋白组(E组),50mg骨蛋白组(F组).A组大鼠灌胃2.5ml/d生理盐水,其它各组大鼠2.5ml/(kg?d)肌肉注射地塞米松,2次/周,共6周造成骨质疏松模型.之后,A和B组灌胃5ml/d生理盐水,C组灌胃300ms/(kg?d)接骨7厘片,D,E,F组分别灌胃200ms/(kg?d),100ms/(kg?d),50ms/(kg?d)猪骨蛋白.12周后取血测AKP和OST水平.结果:c,D,E,F组AKP和OST水平均比B组明显降低(P<0.05).结论:猪
3、骨蛋白降低骨质疏松模型大鼠血中AKP和OST水平.关键词:骨质疏松;大鼠模型;猪骨蛋白;碱性磷酸酶;骨钙素中圈分类号:R一332文献标识码:Bdoi:10.3969/j.issn.1004—437x2011.01.002InfluenceofporcineboneproteintothelevelsofAKPandOSTinserumoftherats,thosteoporosisANYu-hui,YANGLi-jun,SHENYong-jie,SONGDan,HESi—yu(DepartmentofBiochemistryandMol
4、ecularBiologyofBisicMedicalCollege,ghengzhouUnivemi@,Zhengzhou450052,China)Abstract:Objective:Toexploretheinfluenceofporcineboneproteintoalkalinephosphataseandosteoealein.Methods:60Wistarrats,whichareabout3monthesolder,weredividedintonormal(A),physiologicalsaline(B),poss
5、itive(C),200mg(D),100mg(E)and50mggroup(F).ExceptionofAgroup,which2.5mlYdofphysiologicalsalinewaspouredintorat,theratsinothergroupswereinjeetedbydexamethasone,2.5ml(kg?d),2timeseveryweek,total6weeks,theratmodelofosteoporosistobeformed.Then.theratsinAandBgroupswerepouredby
6、physio—logicalsaline,5ml/dperrat.TheratsinCgroupswerepouredby300rag/(kg?d)ofJieguQiliPian.TheratsinD,E,Fgroupswererespectivelypouredinto200ms/(kg?d),100ms/(kg?d)and50mg/(kg?d)ofporineboneprotein.After12weeks,Thelevelsofalkalinephosphatase(AKP)andosteoclacin(OST)inserumwe
7、redetected.Results:ComparingwithBgroup,thelevelsofAKPandOSTinC,D,EandFgroupwereobviouslylower(P<0.05).Conclusion:PorcineboneproteindecreasesthelevelsofAKPandOSTintheSelqlmofratswithosteoporosis.Keywords:osteoporosis;ratmodel;porcineboneprotein;alkalinephosphatase;oste
8、oclacin骨质疏松病是以骨密度和骨强度降低,骨脆性增加,引起骨折,手脚腿抽筋疼痛的骨衰老性疾病….收稿日期:2010.11-20;修订日期:2011-01-26作者简介:安玉会(1952一),男,河南临
