低氧对团头鲂细胞凋亡与抗氧化酶活性的影响

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1、万方数据华中农业大学2015届硕士研究生学位论文mg／L)中，两者的值又有所回升。4．低氧对肝脏抗氧指标的影响对不同低氧组团头鲂肝脏各种抗氧化酶指标的检测结果显示，低氧会导致肝脏中SOD和CAT酶活性、过氧化氢(H202)降低。肝脏中一氧化氮(NO)水平随溶氧浓度的降低及低氧处理时间的延长逐渐增加。肝脏总ROS水平随着低氧处理时间的延长逐渐降低，而复氧及严重低氧时ROS含量回升。关键词：团头鲂；低氧；细胞凋亡；心脏；TUNEL万方数据低氧对团头鲂细胞凋亡及抗氧化酶活性的影响AbstractMegalobramaamb

2、lycephala,alsocalledWuchangfish,isakindofimprovedeconomicfreshwaterfish．Inrecentyears，withthedeteriorationofnaturalenvironmentandhumanactivityintervention,itslivingenvironmentoftenappearedlowoxygen,restrictingthedevelopmentof彪amblycephalabreeding．Inourstudy,the

3、structurechangesofdifferenttissuesofMamblycephalawereobservedafterhypoxiatreatment,andtheactivitiesofantioxidantenzymeswereanalyzedintheliverinhypoxiaandhypoxia-recoveryconditions．Theinfluenceoflowoxygenonmyocardialcellapoptosisandbcl-2expressionwerealsostudied

4、byTUNELandfluorescencequantitativePCR．Additionally,thechangesofMDAcontentandSODactivitywereanalyzedinheartmitochondriaextractedusingsucrosegradientcentrifugationmethod．Themainresultswereasfollows：1．Effectsofhypoxiaonstnl曲lreofdifferenttissuesofM．amblycephalaDif

5、ferenttissues(heart，liver,kidney,muscle，spleen)weresampledandobservedbasedonHEstainingafterseverehypoxictreatment(DO：0．5mg／L)，andtheresultsrevealedthatgreaterinfluenceofhypoxiaontheheartandkidney,whichmusclefmersinheartshowedswellinganddisorderinarrangementinse

6、verehypoxiaconditions，brokencapillarieswereobservedinspleen．However,hypoxiae虢ctsonstructureofmuscle，liverspleenwererelativelylesser．Theresultsshowedbasedonelectronmicroscopethatundermoderatehypoxia(DO：2．5+0．5mgmfor2h，4”，myocardialmitochondrialstructurewerebroke

7、n，mitochondriaWasswellinganddisorder,butinserverhypoxia(DO：O．5mg／L)，somemitochondriawerefractured．2．CardiaccellapoptosiscausedbyhypoxiaInordertofurtherstudyhypoxiae毹ctsonhearttissueofMamblycephala，cardiaccellapoptosisandbcl-2mRNAlevelswereanalyzedbyTUNEL(termin

8、aldexynucleotidyltransferaseTdTmediateddUTPNickendlabeling)andfluorescencequantitativePCRafterhypoxiatreatment．Theresultsshowedthathypoxiainducedcardiaccellapoptosis，andwith