鹅细小病毒抗体胶体金免疫层析检测试纸条的研制

《鹅细小病毒抗体胶体金免疫层析检测试纸条的研制》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、东北农业大学农学硕+学位论文3．2GPV-VP3proteindensityassay⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯243．3Preparationandpurificationofrabbitanti—gooseIgY⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯253．3．1UnpurifiedandpurifiedRabbitanti·gooseIgVvalencyassay⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯。253．3．2Evaluationofpurifiedrabbitanti-gooseIgYpurity⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯

2、⋯⋯⋯。253．3．3Purifiedrabbitanti-gooseIgYdensityassay⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．263．4Preparationandpurificationofrabbitanti-GPV-VP3⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯263．4．1UnpurifiedandpurifiedRabbitanti-GPV-VP3valencyassay⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．263．4．2Evaluationofpurifiedrabbitanti—GPV-VP3purity⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯

3、⋯⋯⋯⋯⋯．．263．4．3Purifledrabbitanti．GPV-VP3densityassay⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯273．5Identificationofcolloidalgold⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．273．5．1Colorobservation⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯273．5．2Ultravioletscanningappraisement⋯⋯⋯⋯⋯⋯⋯．⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯283．5．3Transmi

4、ssionelectronmicroscopescanningappraisement⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯283．6Preparationofimmunitycolloidalgoldteststrip⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．293．6．1ThedeterminationofgoldtaggedproteinoptimizationpHandproteinquantity⋯⋯．293．6．2Colloidalgoldtaggedproteinevaluation⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯303．6．3

5、Initialassemblyandresultsanalysisofthetoststrip⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．313．6．4Theoptimizationofteststrip⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．324Discussion⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．394．1ApplicationprospectsoftheteststripdetectingGPVantibody⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯394．2Thediscussion

6、aboutcolloidalgoldparticle．⋯⋯⋯⋯⋯⋯⋯．⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯394．311地discussionaboutfalsepositiveresult⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯404．3．111圯influenceofelectriccharge⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯404．3．21f1玲influenceonionicstrength⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．414．3．3Theinfluenceontensideandde

7、naturant⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．414．3．4Theinfluenceonconfiningliquid⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．．424．3．5TheinfiuenceonNCmembranehandingliquid⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．434．4Thediscussionaboutsuperperformanceteststrip⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯434．5Thediscussionaboutcontrasttostresults⋯⋯⋯⋯⋯

8、⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．445Conclusion⋯⋯⋯⋯⋯