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ID:33692863
大小:2.42 MB
页数:48页
时间:2019-02-28
《益肾活血提取液对同型半胱氨酸诱导的兔血管平滑肌细胞增殖作用的实验研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、由东大学颈±论文EXPERIMENTAI。STUDYoNEFFECTSoFYISHENHUOXUECAPSULEoNPRoLIFERATIVERABBlTS’VASCULARSMooTHMUSCLECELLSObjectives王NDUCEDBYHCYPostgraduateSupervisorL触Li—pingProLZHANGJi—dongABSTRACT1.ToinvestigatetheeffectsofYishenhuoxueCapsuleonproliferativerabbits’vascularsmoothmusclecellsinducedbyHCY。2.Toinve
2、stigatetheeffectsofYishenhuoxueCapsuleonNF-r,_Bactivation、theexpressionofTGF一131、MCP·1、VCAM一1、PCNAandcellcycles.3.ToexplorethepotentialmechanismofmodulatingtheNF—KBsignaltransductionpathwayonproliferativerabbits’vascularsmoothmusclecellsinducedbyHCY.MethodsVSMCswereculturedbytissueexplantsinvitr
3、oandpuredbythedifferenceofadherentrate.Celluarmorphologyandgrowthnaturewereobservedwithinvertedmicroscope.Afteridentifiedwith鑫一SM—actinbySP,thepurityofcellswasgreaterthan95%,thencellsinlogarithmicphasefrom3generationto6wereusedtotest.ProliferativecellswereinducedbyHCY(1mM),nexttheyweredividedint
4、o6groups—thecontrolgroup(noHCY)、theHCYgroupandfourtreatmentgroups(5000、500、50mg/LYSHXC,5mMfolicacidrespectively),andthentestedafter24hoursculture.TheproliferationofcellsandcellcyclesweredeterminedbyMTTandflowcytometry;theexpressionsofTGF-131、MCP一1、VCAM-1、PCNAandNF。KBactivationweredetectedandanal
5、yzedwithimmunocytochemistryandRT—PCR.Alltestswererepeatedforthreetimes。Thedatawasanalyzedbystatistics.3出东大学颥±论文Resuits1.YSHXCofdifferentconcentrationcouldinhibitcells’proliferation.TheinhibitionandcellcyclesdetectionbyMTI"andflowcytometryshowedthat,intreatmentgroupsthenumberofcellsatGo/Gtstagein
6、creasedgreatly,whilethenumberatSandG2刀隧stagesdecreased;whichwasdifferentfromtheHCYgroup(P7、hichshowedbrownparticlesaswell.TheyweredownregulatedafterYSHXCtreatingfor24hours.DownregulationofTGF一6l、MCP一1、VCAM-1expressionwerecloselycorrelatedwithinhibitionofNF-r,Bactivation(P<0。01).3.RT—PCRanalysisshowedthat,comparedw
7、hichshowedbrownparticlesaswell.TheyweredownregulatedafterYSHXCtreatingfor24hours.DownregulationofTGF一6l、MCP一1、VCAM-1expressionwerecloselycorrelatedwithinhibitionofNF-r,Bactivation(P<0。01).3.RT—PCRanalysisshowedthat,comparedw
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