益肾活血提取液对同型半胱氨酸诱导的兔血管平滑肌细胞增殖作用的实验研究

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1、由东大学颈±论文EXPERIMENTAI。STUDYoNEFFECTSoFYISHENHUOXUECAPSULEoNPRoLIFERATIVERABBlTS’VASCULARSMooTHMUSCLECELLSObjectives王NDUCEDBYHCYPostgraduateSupervisorL触Li—pingProLZHANGJi—dongABSTRACT1．ToinvestigatetheeffectsofYishenhuoxueCapsuleonproliferativerabbits’vascularsmoothmusclecellsinducedbyHCY。2．Toinve

2、stigatetheeffectsofYishenhuoxueCapsuleonNF-r,_Bactivation、theexpressionofTGF一131、MCP·1、VCAM一1、PCNAandcellcycles．3．ToexplorethepotentialmechanismofmodulatingtheNF—KBsignaltransductionpathwayonproliferativerabbits’vascularsmoothmusclecellsinducedbyHCY．MethodsVSMCswereculturedbytissueexplantsinvitr

3、oandpuredbythedifferenceofadherentrate．Celluarmorphologyandgrowthnaturewereobservedwithinvertedmicroscope．Afteridentifiedwith鑫一SM—actinbySP，thepurityofcellswasgreaterthan95％，thencellsinlogarithmicphasefrom3generationto6wereusedtotest．ProliferativecellswereinducedbyHCY(1mM)，nexttheyweredividedint

4、o6groups—thecontrolgroup(noHCY)、theHCYgroupandfourtreatmentgroups(5000、500、50mg／LYSHXC，5mMfolicacidrespectively)，andthentestedafter24hoursculture．TheproliferationofcellsandcellcyclesweredeterminedbyMTTandflowcytometry；theexpressionsofTGF-131、MCP一1、VCAM-1、PCNAandNF。KBactivationweredetectedandanal

5、yzedwithimmunocytochemistryandRT—PCR．Alltestswererepeatedforthreetimes。Thedatawasanalyzedbystatistics．3出东大学颥±论文Resuits1．YSHXCofdifferentconcentrationcouldinhibitcells’proliferation．TheinhibitionandcellcyclesdetectionbyMTI"andflowcytometryshowedthat，intreatmentgroupsthenumberofcellsatGo／Gtstagein

6、creasedgreatly,whilethenumberatSandG2刀隧stagesdecreased；whichwasdifferentfromtheHCYgroup(P

7、hichshowedbrownparticlesaswell．TheyweredownregulatedafterYSHXCtreatingfor24hours．DownregulationofTGF一6l、MCP一1、VCAM-1expressionwerecloselycorrelatedwithinhibitionofNF-r,Bactivation(P<0。01)．3．RT—PCRanalysisshowedthat，comparedw