基于罗丹明类荧光探针的合成与识别性能-研究

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1、万方数据青岛科技大学研究生学位论文SYNTHESISANDRECoGNITIoNPRoPERTIESOFNEWFLUoRESCENTPROBEBASEDONRHODAMINEABSTRACTTherecognitionandsensingofbiologicallyandenvironmentallyimportantspecieshasemergedasasignificantgoalinthefieldofchemicalsensorsinrecentyears．Amongthevariousanalyticalmethods，fluorogenicmet

2、hodsinconjunctionwithsuitableprobesareperferableapproachesforthemeasurementoftheseanalytesbecausefluorimetryisrapidlyperformed，nondestructive，highlysensitive，sutiableforhigh—throughputscreeningapplications，andCanaffordrealinformationonthelocalizationandquantityofthetargetsofinterest

3、．Asafluorophoreandchromophoreprobe，therhodaminefluorochromehasattractedconsiderableinterstfromchemistsonaccountofitsexcellentphotophysicalpropertiessuchaslongabsorption．andemissionwavelength，highmolarextinctioncoefficientandhighfluorescentquantumyieldandSOon．Thefollowingarethemainco

4、ntentofthisdissertation．Inthepartofreview，severalrelatedissuesoffluorescencewereintroduced，includingthebasicconceptoffluorescence，speciesoffluorophores，thedetectionmechanismanditsdevelopmentoffluorescentprobe．Severalfluorescentprobebasedonrhodamine—lactam【weredesignedandsynthesized，

5、andtheirsturetureswereconfirmedby1HNMRand”CNMR，aswellasstudieditsfluorescenceandUVspectrum．Thecompound2-1couldusedasanake—eyefluorescentprobe，whichcoulddetectFe3+inCH3CN，showingthestrongemissionat580nm．BasedonB-Hplotthe1：1bindingmodewasproposed，andthedetectionlimitwasmeasuredtobe5．1

6、8×10。7mol／L(sm≥31．Ⅱ1万方数据基于罗丹明类荧光探针的合成及识别性能研究Fe3+couldherecognizedbycompound2．2i11CHsCNwiththedetectionIimitof1．97×104mol／L(S／N≥3)．Moreimportantly,the2-2-Fe3+complexcouldbeusedasreversiblefluorescentprobetorecognizeF．．Ahovelrhodamine6G．basedsensor2-4wassynthesized，whichexhibitedahigh

7、selectivityforH2P04‘inthepresenceofmanyotheranionsinaCH3CN／H20(5／5，v／v)，andthedetectionlimitwas4．32x10-6mol／L(S／N≥3)．Meanwhilecompound2-4wasalsoappliedtoapH(2．6～4，5)fluorescentsensorinCHaCN-Na3C6Hs07(O．1tool／L；5／5，v／v)buffersolution．Near-infareddye3—1and3-2weredevelopedwithlargestok

8、esshift，andtheirstu