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1、应用与环境生物学报2010，16(4):457~4612010-08-25ChinJApplEnvironBiol=ISSN1006-687XDOI:10.3724/SP.J.1145.2010.00457不同浓度Cd2+对鲤鱼基因组DNA的影响*曹哲明1,2杨健1,2**1（南京农业大学无锡渔业学院无锡214081）2（中国水产科学研究院淡水渔业研究中心内陆渔业环境与资源重点开放实验室无锡214081）摘要鲤鱼（建鲤品种）幼鱼暴露于4个浓度组（0.005、0.05、0.5、5mg/L）的镉离子（C��2+）2��，期间各组的个体均无死亡.用10个RAPD引物对处理后鲤鱼基因组进行扩增
2、，亦未发现有明显的差异扩增条带.但在用甲基化敏感扩增多态性方法研究暴露后鲤鱼肌肉组织的基因组DNA甲基化的变化时发现，5mg/L的C��2+在��2会使鲤鱼基因组DNA中CCGG区域甲基化发生明显的变化.该组个体1mo后出现大量死亡.另外，0.05mg/L和0.5mg/LC��2+组个体基因组DNA中CCGG区域甲基化比例2��内略有下降，甲基化位点没有太大改变.说明鲤鱼幼鱼基因组CCGG区域的胞嘧啶甲基化比例在不同浓度C��2+处理中都比较稳定.低浓度C��2+条件下鲤鱼主要通过甲基化区域的微调减少C��2+的生理毒性.高浓度C��2+处理下，利于基因组DNA甲基化区域发生相当的变化
3、，可能导致一些沉默基因的异常表达.图2表2参31关键词镉；鲤鱼；DNA甲基化；重金属污染CLCX174:S917.4EffectofCadmiumatDifferentConcentrationsonGenomicDNAofCommonCarps*CAOZheming1,2&YANGJian1,2**(1WuxiFisheriesCollege,NanjingAgricultureUniversity,Wuxi214081,Jiangsu,China)(2KeyLaboratoryofEcologicalEnvironmentandResourcesofInlandFisheries,
4、FreshwaterFisheriesResearchCentre,ChineseAcademyofFisherySciences,Wuxi214081,Jiangsu,China)Abstract��he3-month-ol��Jiancommoncarps(Jiancommoncarps(Cyprinuscarpiovar.Jian)wereexpose��tovariousconcentrationsofC��2+(0.005,0.05,0.5,5mg/L)fortwodays,andnoindividualsdiedwithinthetwo-dayexposure.Also,n
5、osignificantlyamplifiedlociwerefoundinbothtreatmentandcontrolfisheswhentenRAPDprimersweredetectedforthedifferenceingenomeDNA.However,whenmethylationsensitiveamplifiedpolymorphism(MSAP)wasappliedtoanalyzemethylationchangesingenomeDNAofmuscle,significantchangesofmethylationinCCGGsitesoccurredinthe
6、fishafter2daysof5mg/LCd2+exposure.Furthermore,mostfishinthisgroupdiedwithinonemonth.Inthegroupsexposedto0.05mg/Land0.5mg/Lfortwodays,aslightdeclineintheproportionofmethylationlociwereobserved,butnoobviouschangeinmethylationpatternincomparisonwiththecontrols.ThisstudyshowsthatthemethyltionofJianc
7、arpinCCGGsitesofgenomeDNAwasrelativelystableduringexposuretovariousconcentrationsofC��2+.AtlowC��2+concentrationtheC��2+physiologicaltoxicitymightbereducedbyJiancommoncarpwithfine-tuninginmethylatedsites,whileathighCd2+conce
