中药活性成分中空纤维细胞捕获及漂浮有机液滴凝固液相微萃取分析

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1、山西医科大学硕士学位论文AbstractAnovelstrategybasedonthehollowfibercellfishingwithchemicalanalysis(HFCF．CA)waSproposedandusedforscreening，fishingandanalyzingbioactiVecomponentsgrouPintraditionalChinesemedicines(TCMs)．Withthenovelmethod，activecomponents，whichpassedthroughtheporesofporoushol

2、lowfiberandcombiningwithlivingcellacceptorlocatedinsideofthehollowfiberlumen，couldbescreenedandfishedfrompH7．4phosphatebufferofTCMsextractionsolution·Subsequently，theactivecomponentsbindingtothecellweredesorbedbycertainsolventsorsolutionsforchemicalanalysis．Inthiswork，anovelmet

3、hodbasedonthehollowfibercellfishingwithhighperformanceliquidchromatography(HFCF-HPLC)hasbeendevelopedandintroducedforscreeningandanalyzingbioactivecomponents2roupoflignansinSchisandrachinensis(Turcz)BaillandcoumarinsinFructus@溯f．乃“ctusPsoraleae．Thesurfacepropertiesofhollowfiber

4、withcellwerecharacterized．thenonspecificbindingsbetweenactivecentersofhollowfiberandthecomponentswereinvestigated，andtherepeatabilitiesofthelignansandcoumannsbioactivecomponentsgroupsscreenedandanalyzedbyHFCF-HPLCweretested．Bycomparingtheretentiontimeswiththestandards，thesevera

5、lactivecomponentsstructuresoflignansandcoumarinsscreenedfromTCMswereidentified．IntheHFCF．HPLC，thesamplecleaning-upandactivityscreenmgwerecarriedoutsimultaneously，whichcouldavoidthecomplicatedprocessofsamplepurification·Thebioactivecomponentsgroupscreeningandseparatingprocesswer

6、eperformedseparately，whichcouldmeetrespectivelytheconditionsofdrug’screeningandchromatographicanalysing．TheresultsdemonstratedthatHFCF·HPLCisasimple，fast．effectiveandreliablemethodforscreeningandanalyzingbioactivecomponents，anditcanbeextendedtoscreenotherbioactivecomponentsgrou

7、pinTCMs．Inthispaper，theextractionmechanismofsolidificationofsuperamoleculeorderingassemblyliquidphasemicroextraction(SSMOALPME)wasproposedbasedonchangeintheUVspectrumsoflignancompoundswithoutandafterthesolidificationoffloatingorganicdropliquidphasemicroextraction(SFODLPME)．TheS

8、SMOALPMEcoupledwithhighperformanceliquidchromatography