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1、促进骨折愈合的作用更强。关键词：骨折、愈合、胰岛素样生长因子Ⅰ、转化生长因子-β2、联合应用2CombinedapplicationofexogenousTGF-β2andIGF-ⅠonfracturehealinginratsAbstractObjective:Comparativeobservationofexogenoustransforminggrowthfactor-β2(TGF-β2),insulin-likegrowthfactorⅠ(IGF-Ⅰ)andthecombinedeffectsoftwogrowth
2、factorsduringfracturehealinginratsunderapplication.Method:(1)120SDratswererandomlydividedinto4groups.Buildtherighttibiafracturemodel.Agroupoflocalfractureafterinjectionofexogenousinsulin-likegrowthfactorⅠ(IGF-I),Bgroupoflocalfractureafterinjectionofexogenoustransfo
3、rminggrowthfactorbeta2(TGF-beta2),Cgroupoflocalfractureafterinjectionofexogenousinsulin-likegrowthfactorⅠandtransforminggrowthfactorbeta2(TGF-beta2),Dgroupwastheblankcontrolgroup.(2)At2and4weeks,eachgroupwererandomlyselectedsixrats,observethebonehardtissuegrowthofn
4、ewborn.(3)At2、4and6weeks,sixratswererandomlyselectedbyX-rayobservationoffracturehealing,thecallusgrowth,parallelimagingscore.(4)Eachgroupat1and3weeks,sixratswererandomlyselectedwerestainedwithHEfromcallustissue,toobservethecalluscells.(5)Eachgroupin6weeks,sixratswe
5、rerandomlyselectedfortestineachgroup,threepointbendingbiomechanics.Results:Generalobservation:Aftertwoweeks,smallgroupscanbepalpableandnewbornhardtissue,AgroupandDgroupnascenthardtissuehardnessisrelativelysoft,newbornhardtissuemorefibrousbonecallustissue,asmallamou
6、ntofcartilagetissuegrowth.GroupBandGroupCisrelativelynascenthardtissueAlargergroupandDgroup,butCgrouplargerandmorerigidhardtissue,includingcartilagetissueastheCgroup,groupBcartilageandfibroustissuealmost.Fourweeksaftersurgery,D、A、B、Cgroupsincreasingrangeofhardtissu
7、e,increasingthehardnessofeachgroupofhardtissue,mostlynewbornbonebonecallus.Imagingobservationandrating:thesecondweekaftersurgery,fourgroupswereclearlyvisiblefractureline,eachgrouphadalittleoutsideofcallusformation,Cexternalcallusrelativelyhigh.Afterthefourth,sixwee
8、ks,fourgroupsweresignificantlyoutsidethecallusformation,D、A、B、Cexternalcallusincreases,radiographicscoreincreases.Histologicalobservation:Afteron
