灯盏细辛预处理对大鼠局灶性脑缺血再灌注后细胞凋亡与socs-3表达影响的实验分析

《灯盏细辛预处理对大鼠局灶性脑缺血再灌注后细胞凋亡与socs-3表达影响的实验分析》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、sacrificedinbatchforobtainingtheirbraintissue，andthentheirbraintissuewouldbecutintofoursections，oneforthewatercontentinbrainmeasuredbywet／dryweight，onefortheapoptoticcellsmeasuredbyTUNEL，oneforthecontentofSOCS一3measuredbyRT—PCRandoneforpathologicalstain．Thesamemethodwasusedtod

2、ealwiththefightbraintissueoftheratsinSG．Results：1)Praxiologyscorecomparison：therewasnoneurologicalfunctionaldefectinSG，neurologicalfunctionaldefectexistbothinCGandEGandtheirneurologicalfunctionalscoresineverytimepointwereremarkablydifferent(P<0．05)．2)Thewatercontentinbrain：the

3、watercontentintheipsilateralbraintissueoffocusinCGandEGbegantoincreaseat6h，andreachedattheirpeakat24h．ButthedegreeofthisincreasewaslowerinEGcomparedwithCGrats(P<0．05)．3)Pathologicalchanges：At24hafteroperation，therewasnopathologicalchangetookplaceinSG，whileserioustissuedestruct

4、ioninischemicregion，intercellularspaceraritas，celluarswelling，nucleusminification，disappearanceandinflammatorycellsinfiltrationwerefoundinCG，andintheEGrats，thepathologicfeatureswerepresentedmuchslighterthanCG，intercellularspacewerefoundalitterbroaden，andthedegreeofcelldegenera

5、tionwerepresentedmuchslightercomparedwithCG，includingpartofcellsswellingandunclearnucleusandendochylema．4)Theapoptosismensurationofneurocyte：ItWasobservedunderlightmicroscopybyTUNEL．Theapoptoticcellswereoccasionallyfoundinshamoperationgroup，whiletheapoptoticcellsinCGandEGbegan

6、toincreasegraduallyafterreperfusion，andreachedattheirpeakat24h，thendecreasegradually．Bycomparison，the4distributionofapoptoticregionandthenumberofapoptoticcellsinEGislessthanCG(P

7、ofSOCS-3increasedsignificantlyattheearlystage，reachedthepeakat24h，andthenreducedgradually．Butafter7dreperfusion，theexpressionwasstillhigherthantheexpressivecontentofSOCS一3inEGWashigherthanthatinCGateverytimepoint(P

8、bymodifiedthreadembolismmethodiseasytooperateandSUCCESS，SOiti