周围神经损伤神经干细胞移植的mri活体示踪分析

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1、中山大学硕士论文ABSlj气RCTMRimaginginvivotrackingofNeuralStemCells‘_●‘-一●transplantedIIIperipheralnerVemlUryl,IL-一-BackgroundMajor：MedicalImaging&NuclearMedicineName：CHENGLi．naSupervisor：AssociateProf．SHENJunABSTARCTPeripheralnervemjury(PNI)isacommontraumaticdiseasewitha

2、highpotentialofdisabilitily．Itsearlyandaccuratediagnosisisofgreatbenefitforthetheraperyandimprovementoftheprognosis．Magneticresonanceimaging(MⅪ)hasbecometheperefereddiagositcchoiceintheperipheralneuropathywithregardtoitshighresolutionofsofttissueandabitlityofnon

3、invasive，directlyvisualizationoftheperipheralnervesandwithoutionisingradiation．Neuralstemcells(NSCs)provideanewapproachforfunctionalreconstructionofnervoussysteminjury．TissuereplacementandtransgenetherapybyNSCstransplantsiscurrentlybeinginvestigatedasapromisinga

4、pproachandbecomemainfocusofmodemregenerativemedicine．Forthestemcelltransplantation,amethodthatcaninvivomonitorthetissuebiodistribution,differentiationandfateofthesecellsinrealtimeandrepeatedlyandnoninvasivelyaregreatlyneeded．Astothisaspect，cellularimagingbasedon

5、MRIprovidestheabilitytoinvivomonitorengraftmentoftransplantedstemcellsnon—invasivelyandbecomeadesirablechoice．Atpresent，thefeasibilityofMRimaginginvivotrackingofNSCstransplantedinperipheralnervemjuryremainsunclear．V孛囊大学矮±论文A转S撇CTObjectives1．Toinvestigatethefeasi

6、bilityandsafetyofinvitrolabelingNSCsbyusingstandardcontrastagents(Gd—DTPA)incombinationwithfluorescencesubstance(PKH26)．2．Toevaluatetheablititytomonitorthedistribution,migrationandfateofNSCstransplantedinperipheralnerveinjuryinvivowithMRIandthedurationoftrack迦．3

7、．ToinvestigatethepossibilityofpromotingrepairofperialnerveinjurywithNSCstransplantionandidentifytheunderlyingmechanism．Materialsandmethods1．CultureandcharacterizationofNSCsEmbryosweredissectedatgestationaldayE16-E18formpregenentrabbits．ThebrainswereremovedandNSC

8、swere洳ked，culturedandpurifiedbyusingthemethodofsuspensioncellcultivation．TheobtainedNSCswereindentifiedandcharacterizedbyusingimmunofluorescencestainingforNestinandNS