单核细胞表达人secp2转基因小鼠的构建及转基因小鼠脑微血管生成分析

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1、万方数据ofmRNAsisolatedfrommonocytes30patientsand26healthycontrolsubjectsinordertofindoutthegeneswhichexpressdifferentlybetweenthem．Basedontheseresults，theaimthisstudyistoinvestigatethesignificanceabnormalexpressiongeneSeeP2inperipheralbloodmonocytesderivedfromAlzheimer’Sdiseasep

2、atients．SeeP2isamemberthefamilycysteine—richproteinscalledSeeP2一likemolecules．SeeP2issecretedintothemediumbyculturedadipocytesandcirculatesinplasma，indicatingthatitisasecretoryproductadiposetissue．However,circulatingmonocytesandmacrophagesinparticularseemtoberesponsibleforSee

3、P2productioninhuman．Intriguingly,recentstudiesinhumansshowaverystrongandconsistentassociationbetweenSeeP2andinflammationand／orinflammatorydiseases．Atpresent，thefunctionSeeP2iscomplexandnotyetfullyunderstood，butitshi曲expressionmonocyteaffectingangiogenesisisthequestionweintere

4、stedOurresultsshowthatSeeP2hasinhibitoryeffectontheformationhumanbrainmicrovascularinvitro．Whetherthisinhibitioneffectonbrainangiogenesisinvivoremainstobefurtherexplored．Inthispaper,inordertostudyhumanSeeP2(hSecP2)onbrainmicrovascularendothelialangiogenesis，weusedanoveltransg

5、enicmousemodel(humanizedSeeP2mice)，ithashighexpressionmonocyte—specifichSecP2．Buildingandusingthisanimalmodel，wecanverifytheresultsinvitroexperimentsinvivo，studyinghumanSeeP2totheeffecthumanbrainmicrovascularendothelialcellsonangiogenesis．Asaresult，thisthesisintendstostudyinv

6、ivoApmetabolismstudyandcerebralmicrovascularangiogenesisSeeP2inperipheralbloodmonocytesderivedfromAlzheimer’Sdisease．Methods1、Weconstructedrecombinantlentiviralplasmid，obtainedpurifiedhightitervirusparticlespecificallytoSeeP2geneover-expressed．(1)Weusedlentivirusexpressionvec

7、tor,constructedthehi曲specificitySeeP25万方数据gelleexpressionplasmid，preparationwithhighfitcrsoflentivirusparticle8·(2)Weused1entivimsparticlestransfectionmonocytesTHP一1，establishedSecP2genehighexpressionmonoclonalcellline·2、SecP2highexpressioneffectsonHBMECangiogenesis·Angiogene

8、sisassayWeplatednormalHBMECwithRecombinantH啪锄SeeP2DroteinorSeeP2gene