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ID:33370185
大小:3.91 MB
页数:32页
时间:2019-02-25
《单核细胞表达人secp2转基因小鼠的构建及转基因小鼠脑微血管生成分析》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、万方数据ofmRNAsisolatedfrommonocytes30patientsand26healthycontrolsubjectsinordertofindoutthegeneswhichexpressdifferentlybetweenthem.Basedontheseresults,theaimthisstudyistoinvestigatethesignificanceabnormalexpressiongeneSeeP2inperipheralbloodmonocytesderivedfromAlzheimer’Sdiseasep
2、atients.SeeP2isamemberthefamilycysteine—richproteinscalledSeeP2一likemolecules.SeeP2issecretedintothemediumbyculturedadipocytesandcirculatesinplasma,indicatingthatitisasecretoryproductadiposetissue.However,circulatingmonocytesandmacrophagesinparticularseemtoberesponsibleforSee
3、P2productioninhuman.Intriguingly,recentstudiesinhumansshowaverystrongandconsistentassociationbetweenSeeP2andinflammationand/orinflammatorydiseases.Atpresent,thefunctionSeeP2iscomplexandnotyetfullyunderstood,butitshi曲expressionmonocyteaffectingangiogenesisisthequestionweintere
4、stedOurresultsshowthatSeeP2hasinhibitoryeffectontheformationhumanbrainmicrovascularinvitro.Whetherthisinhibitioneffectonbrainangiogenesisinvivoremainstobefurtherexplored.Inthispaper,inordertostudyhumanSeeP2(hSecP2)onbrainmicrovascularendothelialangiogenesis,weusedanoveltransg
5、enicmousemodel(humanizedSeeP2mice),ithashighexpressionmonocyte—specifichSecP2.Buildingandusingthisanimalmodel,wecanverifytheresultsinvitroexperimentsinvivo,studyinghumanSeeP2totheeffecthumanbrainmicrovascularendothelialcellsonangiogenesis.Asaresult,thisthesisintendstostudyinv
6、ivoApmetabolismstudyandcerebralmicrovascularangiogenesisSeeP2inperipheralbloodmonocytesderivedfromAlzheimer’Sdisease.Methods1、Weconstructedrecombinantlentiviralplasmid,obtainedpurifiedhightitervirusparticlespecificallytoSeeP2geneover-expressed.(1)Weusedlentivirusexpressionvec
7、tor,constructedthehi曲specificitySeeP25万方数据gelleexpressionplasmid,preparationwithhighfitcrsoflentivirusparticle8·(2)Weused1entivimsparticlestransfectionmonocytesTHP一1,establishedSecP2genehighexpressionmonoclonalcellline·2、SecP2highexpressioneffectsonHBMECangiogenesis·Angiogene
8、sisassayWeplatednormalHBMECwithRecombinantH啪锄SeeP2DroteinorSeeP2gene
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