高龄供体安全性及肾缺血再灌注损伤对高龄大鼠的影响研究

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1、donorkidneyonrenalaUograftlong-termeffectandsafetyevaluationofelderlydonor．Researchmethods：①50cleanhealthymaleSprague--Dawley(SD)rats，30elderly(18"---20months)rats，20young(3"--4months)rats．Theagedratswererandomlydividedintotwogroups：singlekidneygroup(Agedgroupn=15)，ischemia／reperf

2、usiongroup(AgedIRgroupn=15)；Adultratswerealsorandomlydividedintotwogroups：singlekidney(Adultgroupn=10)，Adultischemia／reperfusiongroup(AdultIRgroupn。10)．②WeestablishedagedandadultSDratsrenalischemia／reperfusionandsinglekidneymodeltoexaminebloodureanitrogen(BUN)，serumcreatinine(Scr)

3、，kidneytissuerelatedindexesandtoobservatekidneypathologicalmorphologicalchanges一③TocomparethedifferenceofBUN，ScrandthekidneymorphologychangebetweenAgedandAdult,AgedmandAdultIR．④．ThegeneexpressionofcytokinessuchasTGF--13，IL-6andCaspase-3mRNAweredetectedbyreal-timeRT-PCRat8wkafteris

4、chaemia／Reperfusion．Results：1．Thebloodureanitrogen(BUN)ofAgedIRwasslightlylowerthanAduhIRgroup，butnotobviousat4wkafterischemia／reperfusion(9．77Q士O．403VS8．149-J：1．953，P--0．1359)；thereissignificantdifferencebetweenthesinglekidneygroupstoexamineBUN，theyoungWashigherthantheaged(9．411i

5、-0．804VS6．945：￡-0．824，P<0．0001)．AgedIRgroupwashigherinthedetectionofs睨'umcreatinine(Scr)thanAduRIR(99．78士5．010VS79．53士5．117，P<0．0001)：however,thereiSnostatisticaldifferencemAbs!型Theinfluencestudyofelderlydonorsafetyandischemia／reperfusioninjuryintheagedratsinlevelsofScrbetweentwog

6、roupswithsinglekidneyat4wk(86．76：1：7．330VS85．33+2．172，P=0．5838)2．①11lelevelsofserumBUNdecreasedinagedIRat8wkafterIRIsignificantlycomparetoyoungrats(7．248：∞．929VS8．630士0．830，P=0．0072)，whileserumcreatinine(Scr)washigherthanyoungIRgroupwithsignificantdifference(98．68+3．583VS72．06士于．4

7、62，P<0．0001)．Thereisnostatisticaldif．fbrenceinlevelsofBUNandScrbetweentwogroupswithsinglekidneyat8wk(8．975士1．185VS8．083+0．767，83．05士5．855VS80．03士3．178，PP=O．082>0．05)．②ComparewiththeAdultIR，AgedIRgroupshowedthehighcytokinesmRNAlevelsintissueincluding几缶andTGF-Bat8wk((o．057％士o．042％VS

8、0．011％士0．006％，0．250％士o．1”％VS0．129