mage-a3基因的过表达对人肝细胞癌生物学行为的影响

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1、分类号：R394.3学校代码：10392学科专业代码：100104学号：200901431福建医科大学硕士研究生毕业论文MAGE-A3基因的过表达对人肝细胞癌生物学行为的影响TheeffectofMAGE-A3overexpressiononthebiologicalbehaviourofhepatocellularcarcinoma学位类型：医学硕士所在学院：基础医学院研究生：林青学科、专业：病理学与病理生理学导师：张文敏教授研究起止日期：2010年9月至2012年3月答辩日期:2012年6月8日二○一

2、二年六月万方数据万方数据目录缩略词表···························································1中文摘要···························································3英文摘要···························································5引言···············································

3、············7材料和方法························································10结果··························································24讨论··························································40结论·····················································

4、······44参考文献···························································45致谢···························································49文献综述··························································50万方数据英汉缩略名词对照表（按英文字母顺序排列）英文缩写英文全称中文全称AEC3-amino-9-ethylc

5、arbazole3-氨基-9-乙基咔唑AIApoptosisindex凋亡指数APAmmoniumperoxydisulfate过硫酸铵BCABicinchoninicacid二喹林甲酸DEPCDiethylpyrocarbonate焦碳酸二乙酯DMSODimethylsulfoxide二甲基亚砜ECLEnhancedChemiluminescence增强化学发光试剂FBSFetalbovineserum胎牛血清FCMFlowcytometry流式细胞术GFPGreenfluorescentprotein

6、绿色荧光蛋白HCCHepatocellularcarcinoma肝细胞癌HRPHorseradishperoxidase辣根过氧化物酶LVLentiviralvector慢病毒载体MAGE-A3melanomaantigenA3黑色素瘤抗原A3MTT3-(4,5-Dimethylthiazol-2-yl)-2,5-diphen3-(4，5-二甲基噻唑-2)-2，yltetrazoliumbromide5-二苯基四氮唑溴盐MOIMultiplicityofinfection感染复数ODOpticaldens

7、ity光密度PAGEPolyacylamidegelclectrophoresis聚丙烯酰胺凝胶电泳PBSPhosphatebufferedsaline磷酸盐缓冲液PIPropidiumIodide碘化丙啶PMSFPhenylmethanesulfonylfluoride苯甲基磺酰氟PVDFPolytetrafluoroethene聚偏二氟乙烯RT-PCRReversetranscriptionPCR逆转录聚合酶链式反应SDSSodiumdodecylsulfate十二烷基硫酸钠TBSTris-Buffe

8、redSalineTris-HCL缓冲盐溶液1万方数据TBSTTris-BufferedSalineTween-20含吐温20的TBSTEMEDN,N,N',N'-Tetramethylethylenediamine四甲基乙二胺TrisTris(hydroxymethyl)aminomethane三羟甲基氨基甲烷TUNELterminaldeoxynucleotidyltransferase原位末端脱氧核糖核酸mediated