inhibition of protease activity using pmsf使用pmsf抑制蛋白酶活性

《inhibition of protease activity using pmsf使用pmsf抑制蛋白酶活性》由会员上传分享，免费在线阅读，更多相关内容在应用文档-天天文库。

1、8e506a9fa58ab130269ce1deaf5781be.docPhenylmethylsulphonylfluoride(PMSF)HazardousSubstancesPolicy-AssessmentCHEMICALHAZARDANDRISKASSESSMENTSchoolofBiosciencesNameofsupervisorProf.CMThomasAssessmentNumber*1818SignatureAssessorDrL.BingleDateofAssessment10/07/2001SignatureNotesASchoolC

2、OSHHforminWordisavailableontheSchoolServer.AvailablefromtheHealthandSafetyUnit.GuidanceonmakinganassessmentisgiveninMakingaChemicalHazardandRiskAssessment.GuidanceisalsoavailablefromGuidanceonCompletingtheChemicalHazardandRiskAssessmentForm.Useacontinuationsheettoexpandanysectionof

3、thisforminhardcopyversion.1LOCATIONOFTHEWORKACTIVITYS1012PERSONSWHOMAYBEATRISKListnameswherepossibleDrL.Bingle,DrJ.Hothersall3ACTIVITYASSESSEDInhibitionofproteaseactivityusingPMSF4MATERIALSINVOLVEDAttachcopiesofdatasheet(s)NAMEAMOUNTmax/storedHAZARDRISKPHRASESHAZDATNO***BIOSCIENCES

4、NO***Phenylmethylsulphonyl5gToxicToxicbyinhalation,in1275fluoride(PMSF)contactwithskinandifswallowed.Causesburns.Ethanol2.5litresHighlyHighlyflammable.84flammableCHIPSregulationsapprovedsupplylistclassification.100mMPMSF(solutionin2mlHarmfulandHarmfulbyinhalation,inethanol).highlyc

5、ontactwithskinandifflammableswallowed.Highlyflammable.5INTENDEDUSE**Givebriefdetailsandattachprotocol/instructionsPMSFaddedtobufferusedfordissolving/dilutingproteinsamplesasaproteaseinhibitor.Workingconcentrationtypically1mM.StocksolutionofPMSF(100mMinethanol)storedin0.1mlaliquots.

6、6RISKStoHEALTHandSAFETYfromINTENDEDUSEFrompersonalexposureorhazardousreactions.RefertoOELs,flashpoints,etc.,asappropriate.Arepregnantwomen,breast-feedingmothersespeciallyatrisk?PMSF:Contactwithskinoreyes,orinhalationofpowder,couldoccurduringpreparationofstocksolution.Ethanol:Accide

7、ntalspillageofethanolfromstockbottlecouldresultinafirerisk.100mMPMSFsolution:Contactwithexposedskincouldoccurduringuseofstocksolution.8e506a9fa58ab130269ce1deaf5781be.doc7CONCLUSIONSABOUTRISKSIslevelofriskacceptable?Canriskbepreventedorreducedbychangeofsubstance/procedure?Arecontro

8、lmeasuresnecessary?Risksan