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ID:33167492
大小:3.65 MB
页数:68页
时间:2019-02-21
《sd大鼠优裕胎教和地震胎损的比较实验研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、成都中医药大学2012届硕士研究生学位论文学位论文SD大鼠优裕搿胎教刀和地震“胎损刀的比较实验研究Comparison-experimentresearchof'’fetuseducation¨fromrichenvironmentand"fetusinjury”fromearthquake刘琴指导教师姓名:韭盘鏖熬授申请学位级别:亟±专业名称:控理..堂论文提交时间:2Q!至生墨县论文答辩时间:至Q】至生§旦二O一二年五月成都中医药大学2012届硕士研究生学位论文中文摘要【目的】1.在中医胎教理论基础上,探索建立SD大鼠优裕胎教和地震胎损环境造模。2.观测优裕胎教和地震胎损环境对大鼠生长发育
2、和行为能力等宏观表征的影响。.3.探索金匮肾气丸对地震恐惧胎损的调护作用。【方法】将46只SD孕鼠随机分为四组,优裕组、地震组、中药组和对照组。优裕组给予优裕环境干预,地震组每天模拟地震恐惧干预,中药组在地震组的基础上加用金匮肾气丸,对照组不给与任何干预,按常规喂养,观测孕鼠孕期的体重和行为变化特征,动态监测子代鼠体重、身长、尾长,张耳、睁眼等生长发育指标,进行翻身、亮盒排便及空地测试等行为测试。用酶联免疫吸附测定方法测定各组子代鼠的血清皮质酮和五羟色胺水平。【结果】l。生长发育指标在不同时间点上优裕组子代鼠体重、身长和尾长均优于对照组,差异有统计学意义,P3、鼠体重、身长和尾长均优于地震组,差异有统计学意义,P4、指标皮质酮水平,地震组最高,优裕组和地震组,对照组和地震组差异具有统计学意义,P5、nvironmentand“fetusinjury”fromearthquake.2.Toobservetheinfluencesfrom‘‘fetuseducation”richenvironment,and“fetus!injury’’fromearthquaketothemacrorepresentation,suchasgrowthandbehaviorabilityofrats.Methods46pregnantSDratsweredividedrandomlyintorichenvironmentgroup,earthquakelgroup,TCMgroupandcontrolgr6、oup.TherichenvironmentgroupwasgavefavorableenvironmentinterventionThr矗tentheratsofearthquakegroupandtheTCMgroupwithsimulatedearthquake{andgavetheTCMgroupfodderswithJinkuishenqipill,withthecontrolgroupdidnotofferanyintervention.Observethechangesofbehaviorandweightofpregnantrat,andmeaswethegrowthindex7、,suchasweight,height,taillengthofoffspring,andexperimentaltest,such鹤Turn-overTeStjOpen-fiddTestandLightBoxDefecation.Determinatethehormonelevelbyenzyme。linkedilxRlll.mesorbentassay.Results1.Growthinde
3、鼠体重、身长和尾长均优于地震组,差异有统计学意义,P4、指标皮质酮水平,地震组最高,优裕组和地震组,对照组和地震组差异具有统计学意义,P5、nvironmentand“fetusinjury”fromearthquake.2.Toobservetheinfluencesfrom‘‘fetuseducation”richenvironment,and“fetus!injury’’fromearthquaketothemacrorepresentation,suchasgrowthandbehaviorabilityofrats.Methods46pregnantSDratsweredividedrandomlyintorichenvironmentgroup,earthquakelgroup,TCMgroupandcontrolgr6、oup.TherichenvironmentgroupwasgavefavorableenvironmentinterventionThr矗tentheratsofearthquakegroupandtheTCMgroupwithsimulatedearthquake{andgavetheTCMgroupfodderswithJinkuishenqipill,withthecontrolgroupdidnotofferanyintervention.Observethechangesofbehaviorandweightofpregnantrat,andmeaswethegrowthindex7、,suchasweight,height,taillengthofoffspring,andexperimentaltest,such鹤Turn-overTeStjOpen-fiddTestandLightBoxDefecation.Determinatethehormonelevelbyenzyme。linkedilxRlll.mesorbentassay.Results1.Growthinde
4、指标皮质酮水平,地震组最高,优裕组和地震组,对照组和地震组差异具有统计学意义,P5、nvironmentand“fetusinjury”fromearthquake.2.Toobservetheinfluencesfrom‘‘fetuseducation”richenvironment,and“fetus!injury’’fromearthquaketothemacrorepresentation,suchasgrowthandbehaviorabilityofrats.Methods46pregnantSDratsweredividedrandomlyintorichenvironmentgroup,earthquakelgroup,TCMgroupandcontrolgr6、oup.TherichenvironmentgroupwasgavefavorableenvironmentinterventionThr矗tentheratsofearthquakegroupandtheTCMgroupwithsimulatedearthquake{andgavetheTCMgroupfodderswithJinkuishenqipill,withthecontrolgroupdidnotofferanyintervention.Observethechangesofbehaviorandweightofpregnantrat,andmeaswethegrowthindex7、,suchasweight,height,taillengthofoffspring,andexperimentaltest,such鹤Turn-overTeStjOpen-fiddTestandLightBoxDefecation.Determinatethehormonelevelbyenzyme。linkedilxRlll.mesorbentassay.Results1.Growthinde
5、nvironmentand“fetusinjury”fromearthquake.2.Toobservetheinfluencesfrom‘‘fetuseducation”richenvironment,and“fetus!injury’’fromearthquaketothemacrorepresentation,suchasgrowthandbehaviorabilityofrats.Methods46pregnantSDratsweredividedrandomlyintorichenvironmentgroup,earthquakelgroup,TCMgroupandcontrolgr
6、oup.TherichenvironmentgroupwasgavefavorableenvironmentinterventionThr矗tentheratsofearthquakegroupandtheTCMgroupwithsimulatedearthquake{andgavetheTCMgroupfodderswithJinkuishenqipill,withthecontrolgroupdidnotofferanyintervention.Observethechangesofbehaviorandweightofpregnantrat,andmeaswethegrowthindex
7、,suchasweight,height,taillengthofoffspring,andexperimentaltest,such鹤Turn-overTeStjOpen-fiddTestandLightBoxDefecation.Determinatethehormonelevelbyenzyme。linkedilxRlll.mesorbentassay.Results1.Growthinde
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