bmp15、bmp4基因在不同绵羊群体中的多态性分析及其与产羔数的相关性研究

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1、河北农业大学硕士学位论文BMP15、BMP4基因在不同绵羊群体中的多态性分析及其与产羔数的相关性研究姓名：任立坤申请学位级别：硕士专业：动物遗传育种与繁殖指导教师：刘月琴20120527StudiesonPolymorphismofBMP4BMPl5GeneandItsCorrelationwithLitterSizeinSheepPopulationsPostgraduate：RenLiKunMajor：AnimalGeneticsandBreedingDirection：RegulationofAnimalBreedingTutor：LIUYueqin(Professor)Abstr

2、actInthisstudy,Wechoosebonemorphogeneticprotein4andbonemorphogeneticprotein15弱thecandidategone．Geneticvariationofbonemorphogeneticprotein4∞MP4)gonesequencewasanalyzedbyPCR-SSCP，andthatofBMPl5byPCR-RFLP、PCR_SSCPmethodsinSmallTailedHanSheep，PollDorsetsheep，andtheirFIhybridsheepandF2hybridsheep，stat

3、isticalanalyzedtherelationshipbe时e∞thesitesofdifferentgenotypesandIittersize．、№aimedtoobtainthecorrespondingmoleculargeneticsinfonnationindifferentsheepbreeds，verifythegeneticfeaturesofbonemorphogeneticprotein4andbonemorphogeneticprotein15，andprovideaneffectivemolecularmarkerfortheassistedselecti

4、onaboutthehighprolificacytraitssheepandprovidethebaSisforselectioncrossbreedingtechnologyaboutmultiplebirthsmeatsheep．Ⅵ，edetectthesinglenucleotidepolymorphismofBNml5FecXusitsbyPCR．RFLPandPCR．SSCPtechnologyin402offourdifferentsheepbreedssuchasSmallTailcdHansheep，PollDorsetandthehybridgenerationofS

5、mallTailedHansheepandPollDorset．rnleresultsshowedthattheB2Mutationwasnotdetectedinthatfoursheepgroups蠲itWaSdetectedinBelclareandCambridge．ItshowedthatthenaturalincidenceofB2MutationofBK口15wasverylowinthatfoursheepbreeds．ThesinglenucleotidepolymorphismofBM呼4goneWasdetectedbyPCR．SSCPin335offourdiff

6、erentsheepbreedssuch勰SmallTailedHanowes．11leresultsindicatedthatthealldefrequencyofBand+inSmallTailedHartSheepwere0．167and0．833．genotypofrequencyofBB，B+and++wereO．010，0．297and0．692，theallelefrequencyofBand+inPollDorsetWere0．404and0．596，genotypefrequencyofBB，B+and++wereO．Ooo，0．808and0．192：iIlFIhyb

7、ridsheep，allelefrequencyofBand+were0．250and0．750，genotypefrequencyofBB，B+and-H-Wer0O．053，0．394andO．553；inF2hybridsheep，allelefrequencyofBand+WerO0．025and0．975，genotypefrequencyofBB，B+and+-I-were0．000，0．050and0．950．Thea