各种限制性内切酶的保护碱基&效果

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1、EnzymeOligoSequenceChainLength%CE2hr20hrAccIGGTCGACCCGGTCGACCGCCGGTCGACCGG81012000000AflIIICACATGTGCCACATGTGGCCCACATGTGGG810120>90>900>90>90AscIGGCGCGCCAGGCGCGCCTTTGGCGCGCCAA81012>90>90>90>90>90>90AvaICCCCGGGGCCCCCGGGGGTCCCCCGGGGGA8101250>90>90>90>90>9

2、0BamHICGGATCCGCGGGATCCCGCGCGGATCCGCG8101210>90>9025>90>90BglIICAGATCTGGAAGATCTTCGGAAGATCTTCC81012075250>90>90BssHIIGGCGCGCCAGGCGCGCCTTTGGCGCGCCAA81012005000>90BstEIIGGGT(A/T)ACCC9010BstXIAACTGCAGAACCAATGCATTGGAAAACTGCAGCCAATGCATTGGAACTGCAGAACCAATGCATTG

3、GATGCAT22242702525050>90ClaICATCGATGGATCGATCCCATCGATGGCCCATCGATGGG88101200>905000>9050EcoRIGGAATTCCCGGAATTCCGCCGGAATTCCGG81012>90>90>90>90>90>90HaeIIIGGGGCCCCAGCGGCCGCTTTGCGGCCGCAA81012>90>90>90>90>90>90HindIIICAAGCTTGCCAAGCTTGGCCCAAGCTTGGG810120010007

4、5KpnIGGGTACCCGGGGTACCCC8100>900>90CGGGGTACCCCG12>90>90MluIGACGCGTCCGACGCGTCG810025050NcoICCCATGGGCATGCCATGGCATG814050075NdeICCATATGGCCCATATGGGCGCCATATGGCGGGGTTTCATATGAAACCCGGAATTCCATATGGAATTCCGGGAATTCCATATGGAATTCCC81012182022000075750000>90>90NheIGGCTA

5、GCCCGGCTAGCCGCTAGCTAGCTAG810120101002550NotITTGCGGCCGCAAATTTGCGGCCGCTTTAAAATATGCGGCCGCTATAAAATAAGAATGCGGCCGCTAAACTATAAGGAAAAAAGCGGCCGCAAAAGGAAAA12162024280101025250101090>90NsiITGCATGCATGCACCAATGCATTGGTTCTGCAGTT122210>90>90>90PacITTAATTAAGTTAATTAACCCTT

6、AATTAAGG81012000025>90PmeIGTTTAAACGGTTTAAACCGGGTTTAAACCCAGCTTTGTTTAAACGGCGCGCCGG81012240007502550>90PstIGCTGCAGCTGCACTGCAGTGCAAACTGCAGAACCAATGCATTGGAAAACTGCAGCCAATGCATTGGAACTGCAGAACCAATGCATTGGATGCAT814222426010>90>900010>90>900PvuICCGATCGGATCGATCGATTCG

7、CGATCGCGA81012010002510SacICGAGCTCG81010SacIIGCCGCGGCTCCCCGCGGGGA8120500>90SalIGTCGACGTCAAAAGGCCATAGCGGCCGCGCGTCGACGTCTTGGCCATAGCGGCCGCGGACGCGTCGACGTCGGCCATAGCGGCCGCGGAA2830320101005075ScaIGAGTACTCAAAAGTACTTTT81210752575SmaICCCGGGCCCCGGGGCCCCCGGGGGTCCC

8、CCGGGGGA6810120010>90101050>90SpeIGACTAGTCGGACTAGTCCCGGACTAGTCCGCTAGACTAGTCTAG8101214101000>90>905050SphIGGCATGCCCATGCATGCATGACATGCATGCATGT81214001002550StuIAAGGCCTTGAAGGCCTTCAAAAGGCCTTTT81012>90>90>90>90>90>90XbaICTCTAGAGGCTCTAGAGCTGCT