红景天苷对大鼠脑缺血再灌注损伤的保护作用

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1、———————————————型必芏趔f兰li垫塞ProtectiveEffectandItsMechanismofSalidrosideagainstCerebralIschemia·ReperfusionInjuryinRatsAbstraetAstheadvancementofthemechanismaboutcerebralischemia—reperfusioninjury．newphysiopathologicmechanismhasbeenrecognizedAlotofinvestigati

2、onshavebeenconfirmedthatreperfusionafteracutefocalcerebralischemiacarlaggravatetheinjuryAtpresent，themostextensivelyanddeeplystudiedaspectsarefreeradicleinjuryandtheexpressionoftheproteinassociatedwithapoptosisRecently，salidrosidewhichisthemaineffectiveing

3、redientofintegripetalrhodiolaherbhasbeenreportedpossessingextensivepharmacologicalactionsThemaineffectsofsalidrosideareenhancingmemory，protectingcardiovascularsystem，enhancingimmunity，resistinghypoxiaandsoonMorestudieshavebeenfocusedontheprotectiveeffectof

4、salidrosideagainstcardiovasculardisease，however,theneuroprotecdveeffectofsalidrosideespeciallyinvivowaslessstudiedThepurposesofthepreseatstudyv／ergtoevaluatetheeffectsofsalidrosideonfreeradicatscavengerandtheproteinexpressionassociatedwithapoptosisinMCAOra

5、tsPartIOBJECTIVEToinvestigatetheeffectsofsalidrosideoncerebralischemia-reperfusioninjuryinmiddlecerebralarteryocclusive(MCAO)ratsMETHODSFocalcerebralischemiainratswasproducedby15hocclusionofthemiddlecerebralarteryand24hreperfusionSalidroslde(25、5、10mg’kg“)

6、wasadministeredintravenouslyimmediatelyafterocclusionandreperfusionrespectivelyTheneurologicdeficitscorewasinvestigatedaccordingtoZea-LongalsStandardTheinfarctareawasassessedwithsoftwareImageproPlus60afterTTCstainingRESULTSComparedwiththemodelgroupsalidros

7、ide(5、10mg／kg)cansignificantlydecreasetheneurologicdeficitscoreandreducetheinfarctareaofthebraininMCAO—I／RratsCONCLUSIONSalidrosidecouldprotectMCAOratsfromcerebralischemia—reperfusioninjuryPartIIOBJECTIVEToexploretheeffectsofsalidrosideonactivitiesofGSH—PX

8、andiNOSaswellastheexpressionofapoptosisrelatedproteininMCAO-I／RratsMETHODSSDratssvererandomlydividedintoshamgroup，modelgroupandsalidroside5mg／kggroupTheactivitiesofinduciblenitricoxidesynthase(iNOS)andglutath