雌激素对卒中后抑郁大鼠海马中bdnf2ftrkb2fcreb信号的影响

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1、万方数据温州医科大学硕士学位论文2．糖水偏爱试验行为学糖水偏爱试验行为学结果：①PSD模型完成(基线)各组大鼠糖水偏爱度两两比较无统计学意义(P>0．05)；⑦雌激素干预1周后，雌激素+K252a组和雌激素+U0126组分别与雌激素组比较，大鼠的糖水偏爱度显著降低伊<0．05)；③雌激素干预2周后，与雌激素组比较，雌激素+K252a组的大鼠糖水偏爱度有显著降低(P

2、a、U0126、人工脑脊液和DMSO后，K252a组和U0126组分别与人工脑脊液组相比，pTrkB／13．actin、Trka／f1．actin和pCREB／CREB的灰度比值水平均显著下降(P

3、行为学的评估，成功复制实验大鼠卒中后抑郁模型。2．在PSD模型的基础上，给予雌激素T预后，多种行为学观察结果表明雌激素可以改善脑卒中大鼠抑郁样症状。3．雌激素对PSD大鼠海马中BDNF，nl(B／CREB信号通路表达起到上调作用。通过阻断TrkB和CREB的表达，雌激素对激活BDNF的蛋白表达明显起到抑制作用，其行为学评分显著减少，间接表明了BDl师仃球B／CREB信号通路存在内在相互联系，揭示PSD的分子机制之一。关键词雌激素、海马、卒中后抑郁、酪氨酸激酶B、脑源性生长因子、cAMP应答元件结合蛋

4、白4万方数据温州医科大学硕士学位论文EstrogenAdministrationAttenuatesPost-strokeDepressionbyEnhancingCREB／BDNF／TrkBSignalingintheRatHippocampusAbstractobiective：Weestablishedthemodelofpost—strokedepression(PSD)，toresearchtheeffectofestrogenonbrain—derivedneurotrophicfact

5、or(BDNF)，anditsreceptortyrosinekinaseB(TrkB)anddownstreamsignalmoleculesincludingcAMPresponseelement-bindingprotein(CREB)loopproteinlevelexpressioninthehippocampusofPSDratS．Methods：1．Thisty—sixSprague—Dawleyfemaleratsweighingbetween225and2559wererandom

6、lydividedintocontrolgroup，PSDgroupandestrogengroupintheexperiment．Allratswereovariectomized．Controlgroupwerenotgivenotherstress．PSDgroupsubjectedtoleftmiddlecerebralarteryocclusion(MCAO)followedbyexposuretochronicunpredictablemildstress(CUMS)forconsecu

7、tive2weeks，injectedsubcutaneouslyoilforconsecutive2weeks．EstrogengroupweretreatedsubcutaneouslywithlOpg1713-estradiol(E2)forconsecutive2weeksinPSDrats．Theseanimalsdevelopedrobustdepression—likebehaviorusingbothopen—fieldtest，sucrosepreferenceindextesta

8、ndforceswimtest．TheprotienlevelexpressionofBDNF／TrkB／CREBsingallinginthehippocampuswereinvestigatedusingImmunohistochemistryandWesternBlotting．2．ThePSDmodelsubjectedtoMCAOfollowedbyexposuretoCUMSandseparation．Forty-fourPSDratswereusedin