血管神经化组织工程骨成骨效果一年观察

《血管神经化组织工程骨成骨效果一年观察》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、硼士掌位论文OneyearobservationsofosteogenesiseffectsontheconstructionofVascularizationandneurotizationoftissue．engineeredbonePostgradute：DaiJinLiangSupervisor：PeiGuoXianABS下RAff0bjectiveThevascularizationandneurotizationproblemisafocalpointinthestudyoftissueengineeredbone．Asthebonetissueengineeringisstud

2、ieddeeplyandgraduallyaimedtotheclinicalapplication，howtoconstructtissue-engineeredbonesimultaneouslywiththereconstructionofblood·supplyandinnervationsbecomesthekeyproblem．Thisexperimentisonthebasisofconstructingthevascularizedandnervalizedtissue。engineeredboneinanimalvivo，throughoneyearobservationa

3、ndassessmentofosteogenesistOdiscusstheeffectofvascularizationandneurotization。Furtherenrichthetheoryofvascularizationandneurotizationoftissue．engineeredbone，andofferthebasisforclinicalapplication．Thisexperimentpurpose：‘1)Toinvestigateoneyearosteogenesiseffectsoflargetissueengineeredbonewrapedupbybl

4、oodsuppliedfascia；《2)Toinvestigatetheosteogenesiseffectsinvivoofdifferentkindsofnerves—tractimplantintolargetissueengineeredbone．MethodsThefirstpartOne-yearobservationoftissueengineeredboneofABSTRACTosteogenesiseffectsacceleratedbybloodsuppliedfasciaflap1、16NewZealandrabbitswereusedfortheexperiment

5、．Autologousboneman'owstromalcells03MSCs)wasobtainedfromtheiliaccrestofrabbitsbyneedleaspirationandthebonemarrowstromalcelIs(BMSCs)wereinducetoosteoblastandwerecultivatedwith多一托P(13--1ricalcium曲osphate，多一誓CP)torepairlargebonedefect(1．5cm)inrabbit。Stworadiuses；thetissueengineeringbonewerewrapedupbybl

6、oodsuppliedfasciainrabbit’Sleftradius，舔thecontrol，thetreatmentwasnotdonetorightradius．2、At6and12monthsafteroperation，grossobservation、X—rayexaminationandhistologicalexaminationwereadoptedtoevaluatetheosteogenesisandvascularize．Statisticsisdealtwi氇theresultingdata,inordertOcomparethereparationofbone

7、defectsarea．ThesecondpartOne-yearobservationoftissueengineeredboneofosteogenesiseffectsafternervetractimplantations1、80NewZealandrabbitswereusedfortheexperiment．Autologousbonemarrowstromalcells(BMSCs)Wasobt