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ID:33012588
大小:3.01 MB
页数:45页
时间:2019-02-19
《血管神经化组织工程骨成骨效果一年观察》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、硼士掌位论文OneyearobservationsofosteogenesiseffectsontheconstructionofVascularizationandneurotizationoftissue.engineeredbonePostgradute:DaiJinLiangSupervisor:PeiGuoXianABS下RAff0bjectiveThevascularizationandneurotizationproblemisafocalpointinthestudyoftissueengineeredbone.Asthebonetissueengineeringisstud
2、ieddeeplyandgraduallyaimedtotheclinicalapplication,howtoconstructtissue-engineeredbonesimultaneouslywiththereconstructionofblood·supplyandinnervationsbecomesthekeyproblem.Thisexperimentisonthebasisofconstructingthevascularizedandnervalizedtissue。engineeredboneinanimalvivo,throughoneyearobservationa
3、ndassessmentofosteogenesistOdiscusstheeffectofvascularizationandneurotization。Furtherenrichthetheoryofvascularizationandneurotizationoftissue.engineeredbone,andofferthebasisforclinicalapplication.Thisexperimentpurpose:‘1)Toinvestigateoneyearosteogenesiseffectsoflargetissueengineeredbonewrapedupbybl
4、oodsuppliedfascia;《2)Toinvestigatetheosteogenesiseffectsinvivoofdifferentkindsofnerves—tractimplantintolargetissueengineeredbone.MethodsThefirstpartOne-yearobservationoftissueengineeredboneofABSTRACTosteogenesiseffectsacceleratedbybloodsuppliedfasciaflap1、16NewZealandrabbitswereusedfortheexperiment
5、.Autologousboneman'owstromalcells03MSCs)wasobtainedfromtheiliaccrestofrabbitsbyneedleaspirationandthebonemarrowstromalcelIs(BMSCs)wereinducetoosteoblastandwerecultivatedwith多一托P(13--1ricalcium曲osphate,多一誓CP)torepairlargebonedefect(1.5cm)inrabbit。Stworadiuses;thetissueengineeringbonewerewrapedupbybl
6、oodsuppliedfasciainrabbit’Sleftradius,舔thecontrol,thetreatmentwasnotdonetorightradius.2、At6and12monthsafteroperation,grossobservation、X—rayexaminationandhistologicalexaminationwereadoptedtoevaluatetheosteogenesisandvascularize.Statisticsisdealtwi氇theresultingdata,inordertOcomparethereparationofbone
7、defectsarea.ThesecondpartOne-yearobservationoftissueengineeredboneofosteogenesiseffectsafternervetractimplantations1、80NewZealandrabbitswereusedfortheexperiment.Autologousbonemarrowstromalcells(BMSCs)Wasobt
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