脓毒症大鼠肺组织线粒体内hmgb1与线粒体损伤关系的实验-研究

《脓毒症大鼠肺组织线粒体内hmgb1与线粒体损伤关系的实验-研究》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、脓毒症；肺脏；线粒体；高迁移率脂蛋白B1；作者：王俊指导老师：邵义明主任医师TherelationshipbetweenthemitochondrialdamageandHMGBlexpressioninmitochondrialinlungtissueofratswithsepsis【Objective】AbstractBydetectedtheexpressionofhi曲mobilitygroupproteinB1(HMGB1)inlungtissueofsepsisratsthroughreal-timefluorescentquantitativePCR，andthedegre

2、eofmitochondriadamageinlungtissueofsepsisratsbyJC一1fluorescenceprobeandconfoeallaserscanningmicroscopy(CLSM)．finalanalyzetherelationshipbetweentheexpressionlevelofHMGB1insidethemitochondriaandthedegreeofmitochondriadamage．Weprobedintothepossiblemechanismofmitochondriadamageinlungtissueofsepsisrat

3、sSOastofindtheeffectivewayoflungprotectioninsepSlS．【Methods】50SDratsofSPFlevel(maleandfemaleinformality,weight180-200grams)，wererandomlydividedintoexperimentalgroupandcontrolgroup，25SDratsineachgroup．Weestablishedsepsismodelbycecalligationpuncture(CLP)intheexperimentalgroup，thecontrolgroupexceptf

4、ornotCLP,othertreatmentwithexperimentgroup．Allgroupswererespectivelyputtodeathinthetimepointof6h，12h，24h，48h，72h．Take100mgfreshlungtissuetoextractthemitochondriaandthendetectimmediatelymitochondrialmembranepotentialbyCLSMandtheexpressionlevelmitochondrialHMGB1byrealtimefluorescentquantitativePCR．

5、【Results】1Comparedwiththecontrolgroup，thelevelofmitochondrialmembranepotentialinlungtissueofsepsisgroupwassignificantlydecreased(尸

6、enweakenat24h，48h，butenhancedmarkedlyat72h．2Comparedwiththecontrolgroup，theexpressionlevelofmitochondrialHMGB1inlungtissueofsepsisgrouphadaremarkableenhancement(P<0．01)．Asthesurgerytimegoneon，theexpressionlevelofmitochondrialHMGB1reachedpeakat12h，thenweakenat24h，48h，butreachedanotherpeakat72hagai

7、n．3TherewaspositivecorrelationbetweenmitochondrialdamagedegreeandexpressionlevelofmitochondrialHMGBlfr=O．709)．【Conclusions】Thereismitochondriadamageinlungtissueofsepsismodel，themostseriousinjuryisat12h，theninjurydeclin