叶酸缺乏通过线粒体途径抑制孕鼠子宫内膜蜕膜细胞凋亡

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1、重庆医科大学硕士研究生学位论文FOLATEDEFICIENCYDECREASESAPOPTOSISOFENDOMETRIUMDECIDUALCELLSINPREGNANTMICEVIATHEMITOCHONDRIALPATHWAYABSTRACTObjective：Itiswellknownthatmaternalfolatedeficiencyresultsinadversepregnancyoutcomes，includingembryonicmalformations,abortion,preterm,lowbirthweightandsoon.Amongthem,therelation

2、shipbetweenfolatedeficiencyandneuraltubedefects(NTD)hasbeenrecognizedbythescholars.Previousstudiesfocusedtheireyesontheeffectoffolatedeficiencyonthefetuswhileignoringitsimpactonthematernalaspects.Inadditiontoaspectsinembryonicdevelopment,maternaluterinereceptivityanddecidualizationofstromalcellsarea

3、lsoveryimportantforasuccessfulpregnancy.Ourpreviousstudyrevealedthattherewasnoeffectoffolatedeficiencyonembryoimplantation,andboththeexpressionofuterinereceptivitymarkergenesandthenumberofimplantationsitesshowednosignificantdifferencebetweenthefolatedeficiencygroupandcontrolgroup.However,theoutcomeo

4、fthefolate-deficientpregnantmicewasnotfavorable,whichindicatesmoreembryolossandsmallerembryovolume.Inthisstudy,weinvestigatedwhetherapoptosisofthedecidualcellswasimpairedunderfolate-deficientconditionsandexploredmaternaluterine4万方数据重庆医科大学硕士研究生学位论文endometriumdecidualizationinthatcondition.Methods:Afo

5、late-deficientpregnantmousemodelandanormaldietpregnantmicemodelwereestablished.Flowcytometry,JC-1detectionandTUNELwereusedtodetectapoptosisofthedecidualcellsisolatedfrompregnantmiceonday7and8.TEMwasusedtoobservethemorphologyofmitochondriaandendoplasmicreticulum.Immunohistochemistrywasusedtodetectthe

6、proteinexpressionandlocationofBaxandBcl2.TheexpressionofBax、Bcl2、cleaved-Caspase3、pro-Caspase3、cytochromecweredetectedusingWesternblot.Immunofluorescenceandlaserscanningconfocalmicroscopewereusedtoobservethereleaseofcytochromecfrommitochondriatocytoplasm.WesternblotandRT-PCRwereusedtodetecttheexpres

7、sionofgenesHoxa10,BMP2,MMP2,MMP9，whichwererelatedtoendometriumdecidualization.Results:Decidualcellsofmiceinthecontrolgroupexhibiteddilationoftheendoplasmicreticulumanddistendedmitochondria,whilethefol