新型非细胞型组织工程人工骨修复骨缺损的实验分析

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1、中文摘要骨支架材料，可用于治疗骨缺损。2BMP具有明显的促进骨形成作用，nHAC／PLA和越AC／PL～CMs．BMP复合支架的骨修复能力明显强于单纯nHAC／PLA和nHAC／P洲CMs支架。3nHAC／PLA和nHAC／PLA／CMs．BMP复合支架可应用临床治疗骨缺损。关键词：组织工程；人工骨；骨缺损；纳米羟基磷灰石；壳聚糖；BMP；胶原英文摘要StudiesonboneDefectRepairedwithNewNon-cellBasedBoneTissueEngineeringArtificialBoneABSTRACTobjective：Nanometer

2、1StheBMPcarriertocurebonedefection．Methods：1Usenanotechhnologytostudyllanoextracellelramatrixsimillarartificialbonestructure．BMP—loadedchitosansustained—releasegelmicrosphereswereprepredviaemulsioncross-linkingtechnology．BMP·-loadedchitosansustained-·releasegelmicrospherecombinednanoex

3、tracellelramatrixdevelopintoNon—cellBasedBoneTissueEngineeringArtificialBone．ItsteriliedviaC060．2Reparedanimelmodelofrabbitsfemoralcondylarbonedefection．3Use4．5Newzealandrabbitofbiaear(2-2．5kg)regardlessmaleorfemale，anddividethemintogroupA，B，andC，15eachgroupAddscaffoldmaterial+5mgBMPto

4、groupAbonedefection；OnlyaddscaffoldmaterialtogroupB；Cgroupwereservedasshamcontrolgroup．Thethreegroupsshouldbeprepredandfedunderthesamecondition，lettingthemactfreely．4Observetherabbits’actionstate，eating，andfeleseveryday．Executethmat4w,8w，12w'doingroughlyobservasion，imagingstudy,histolo

5、gicalanalysisandstatisticallyanalyzed．Bonerecovewereobserved．Results：1Atthefourthweek，wecanseegroupAandBbonedefectionareacoverdautograntmaterialandsurfacefiberandthereisnotissuegrowinCgroup．2Histologicaiobservasion：Incuregroup，weCallseebonescabfomedafterfourweeks，andactioncellsaroundit

6、，roughlyorganizedboneisformedafter8weeks，chitosanscaffoldmaterialisalmostabsordedafter12weeeks，largeosteoblastgrowed，wecanseebonemarrowtissue．Inthecontrastgrouptissuescaffoldiscomplete，fillingtissueiSlittle，osteoblastareincreasingafter8weeeks，andscaffoldwerepartobsorbed，littlevague，ost

7、eoblastdidn’tfillscaffoldarea．Insham3英文摘要controlgroupHEstainingisnogreatchanges，andlittleorganiaedboneandnewboneSCadformed．3Imagingstudy：IncuregroupwecanseelowvaguebonescabformedatbonedefectionaReronemonth．neimagingofregularlowdensitybonescabisclearandcontinuousbonescabisformedaRer2m