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ID:33004981
大小:1.33 MB
页数:40页
时间:2019-02-19
《新型非细胞型组织工程人工骨修复骨缺损的实验分析》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、中文摘要骨支架材料,可用于治疗骨缺损。2BMP具有明显的促进骨形成作用,nHAC/PLA和越AC/PL~CMs.BMP复合支架的骨修复能力明显强于单纯nHAC/PLA和nHAC/P洲CMs支架。3nHAC/PLA和nHAC/PLA/CMs.BMP复合支架可应用临床治疗骨缺损。关键词:组织工程;人工骨;骨缺损;纳米羟基磷灰石;壳聚糖;BMP;胶原英文摘要StudiesonboneDefectRepairedwithNewNon-cellBasedBoneTissueEngineeringArtificialBoneABSTRACTobjective:Nanometer
2、1StheBMPcarriertocurebonedefection.Methods:1Usenanotechhnologytostudyllanoextracellelramatrixsimillarartificialbonestructure.BMP—loadedchitosansustained—releasegelmicrosphereswereprepredviaemulsioncross-linkingtechnology.BMP·-loadedchitosansustained-·releasegelmicrospherecombinednanoex
3、tracellelramatrixdevelopintoNon—cellBasedBoneTissueEngineeringArtificialBone.ItsteriliedviaC060.2Reparedanimelmodelofrabbitsfemoralcondylarbonedefection.3Use4.5Newzealandrabbitofbiaear(2-2.5kg)regardlessmaleorfemale,anddividethemintogroupA,B,andC,15eachgroupAddscaffoldmaterial+5mgBMPto
4、groupAbonedefection;OnlyaddscaffoldmaterialtogroupB;Cgroupwereservedasshamcontrolgroup.Thethreegroupsshouldbeprepredandfedunderthesamecondition,lettingthemactfreely.4Observetherabbits’actionstate,eating,andfeleseveryday.Executethmat4w,8w,12w'doingroughlyobservasion,imagingstudy,histolo
5、gicalanalysisandstatisticallyanalyzed.Bonerecovewereobserved.Results:1Atthefourthweek,wecanseegroupAandBbonedefectionareacoverdautograntmaterialandsurfacefiberandthereisnotissuegrowinCgroup.2Histologicaiobservasion:Incuregroup,weCallseebonescabfomedafterfourweeks,andactioncellsaroundit
6、,roughlyorganizedboneisformedafter8weeks,chitosanscaffoldmaterialisalmostabsordedafter12weeeks,largeosteoblastgrowed,wecanseebonemarrowtissue.Inthecontrastgrouptissuescaffoldiscomplete,fillingtissueiSlittle,osteoblastareincreasingafter8weeeks,andscaffoldwerepartobsorbed,littlevague,ost
7、eoblastdidn’tfillscaffoldarea.Insham3英文摘要controlgroupHEstainingisnogreatchanges,andlittleorganiaedboneandnewboneSCadformed.3Imagingstudy:IncuregroupwecanseelowvaguebonescabformedatbonedefectionaReronemonth.neimagingofregularlowdensitybonescabisclearandcontinuousbonescabisformedaRer2m
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