盐酸罂粟碱对任意皮瓣微循环影响的实验分析

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1、英文摘要“1210)whichslzewasabout0．3cmxO．3cmwastakenrespectivelyinthe4mdayafteroperationwasconservedin4％paraformaldehydeinordertocommitparaffinsectionanddyeingofmmunohiscochemistry．Theflapviabilityinthesetwogroupswasobservedtendaysafterthepediclewascut．5．Theobservationmarker(1)Accordingtothespecificat

2、ionofthetestkit，thecontentofMDAweremeasured．Thequantityoftheproteinintheobtainedflapwasmeasuredwiththetestkitofcoomassielightblue．(2)Inthe10‘“dayafterthepediclewascut，theflapviabilitywasobserved．Theareasofthetotalskinflapandthesurvivalskinflapweredepictedonasulfuricacidpaper．Thepapersonbehalfoft

3、heflapareawereshearedandwereweightedwiththeelectronicscale．Theflapareawasreprensentedbythepaper’Sweight．Thentheflapviabilitywascalculated．(3)Themethodofimageanalysis：Theskingraftsweresampledtoobservenumbersofbloodcapillaryandgrowthofgranulationtissueswithmicroscope．6．Analysisofresults：Differentk

4、indsofresultsweredealedwithbyusingSAS6，l2SOftware．Results：1Flapsurvivalwas89±4％inthetestgroupand63+7％inthecontrolgroup．TherewassignificantIy(p<0．05)differenceinthetwogroups．2Microvesselnumerber．themicrovesselnumerberresultareshowninthetable2andrandomskinflapinthetestgroupshowedsignificantly(p<0．

5、05)increasedthemicrovesselnumerberwhencomparedwiththecontrolgroup．3ThelevelofMDAinthe4仇一一茎查塑兰——————●——-—_——_—_——__—————————●———__————————_————一一一dayafteroDeration：Flapsinthetestgrouphadameanlevelof0．564+0．245％VS．1．108+0．237％inthecontrolgroup(p

6、ps·Conclusion：Inconclusion，papaverineisaseffectivewhenadministeredlocallyintotheflapasinthecaseofsystemmadministration．Bvlocalapplication，itispossibletoavoidunwantedsvstemicsideeffects．WeconcludethatthisprocedurecanbeadaptedeasilytOclinicalpractice，andcanbeareliablealternativeforincreasingflapvi

7、ability．Keywords：skinflap；papaverine；Cuttingpedicleinearlystage；survivalrate；Microcirculatiomischemia--reperfusioninjury；immunohistochemistry6研究论文盐酸罂粟碱对任意皮瓣微循环影响的实验研究前言由于皮瓣自身有血供，并可以根据受区需要切取不同厚度的组织，以修复各种组织器官缺损和畸形，可以更好的恢复机体的功能