不同途径导入vegf基因对缺血皮瓣影响的实验的研究

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1、摘要目的：比较不同途径导入VEGF对大鼠腹部缺血皮瓣的影响，探讨有效的基因导入方法。方法：建立大鼠腹壁下动脉为蒂的缺血皮瓣动物模型，30只SD大鼠随机分成经缺血皮瓣蒂动脉组，皮下肉膜组，对照组，术后7d切取皮瓣，RT—PCR、免疫组化染色及真彩色医学图像分析软件等方法检测VEGF基因的表达、皮瓣皮下血管密度。结果：三组之间互相比较，表明在缺血皮瓣中VEGF基因的表达、血管的密度，蒂动脉组、皮下肉膜组与对照组有显著差异(尸<0．01)，蒂动脉组与皮下肉膜组有差异(尸<0．05)。结论：VEGF基因经皮瓣蒂动脉或

2、经皮瓣肉膜的途径导入均能在缺血皮瓣中有效表达，增加新生血管，改善缺血皮瓣的血供，其中前者更有效。关键词：VEGF基因；治疗途径；缺血皮瓣AbstractABSTRACTObjective：BycomparethedifferenteffectsoftransferringthenakedplasmidDNAencoding121．aminoacidisofonnofVEGFintotheischemicskinflapoftherats，viaarteriousrout，subcutaneousrout，an

3、dcontrolgroup，wetrytoexploreaeffectiveapproachofgenetransferring．Methods：All30Sprague—Dawleyratswererandomlydividedintothreegroups，with10ratsforeachgroup．Theanimalmodelofischemicskinflapwasestablished．TheratsingroupAandgroupBweretreatedwiththenakedplasmidDN

4、Aencoding121-aminoacidisoformofVEGFthatwasinsertedintopcD2．GroupA(pcD2／hVEGFl21，arteriousn=10)，groupB(pcD2／hVEGFl21，Subcutaneous，n=10)，groupC(controlgroup，n=lO)．ThepcD2／llVEGFl21wereinjectedintotheischemicskinflapthroughthepedicleofinferiorepigastriaarteryi

5、ntheratsofgroupA．TheratsofgroupBwerereceivedthepcD2mVEGFl21bytheapproachofinjectingdirectlyintothepanniculuscarnosismuscleoftheischemicskinflap．TheratsofgroupCwerenotreceivedtheadministration．After7days，RT-PCRtechnologyandimmunohistochemistry，colormedicalim

6、ageanalyticalsystem(CMIAS)wereusedtoexaminetheexpressionofVEGFgene，thesubdermalvasculardensityoftheischemicskinflap．Results：TheexpressionofthegeneandsubdermalvasculardensityweremarkedlyincreasedintheflapsofVEGFgene-treatedratsthanthoseinthecontrolgroup俨<0．0

7、1)．Furthermore，theischemicskinflaptreatedwithVEGFgeneviaarteryshowedadifferenceinrespectofenhancingtissueviabilityattheseventhdayafterinjection．Conclusion：TheVEGFgenetherapyisabletoenhancethesurvivaloftheischemicskinflapbyenhancingtheexpressionofthegene，ind

8、ucingangiogenesisandsupplyingmorebloodflowtoischemicskinflap．TheroutofinjectingVEGFgenethroughthepedicleofinferiorepigastriaarteryoftheischemicskinflapenhancedtissueviabilitymoreeffectivethantheroutoft