coq10对cr(ⅵ)诱导的l-o2肝细胞凋亡的干预作用分析

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1、万方数据硕士学位论文英文摘要TheinterventioneffectofCoQl0onCr(Ⅵ)-inducedapoptosisinL一02hepatocytesAbstractobjective：Byfurtherexploringthetoxicmechanismsofhexavalemchromium[cr(VI)]andlookingfortheantidoteforCr(VI)-inducedcytotoxicity,weaimedtoprovideexperimentalevidenceforthepreventionandcureoftheoccupationalhaz

2、ardandthehealthpromotionforthepeopleexposedtoCr(VI)．Methods：啊1esurvivalratesofL一02hepatocytestreatedwithdifferentconcentrationsofCr(VI)andCoQlOweredetectedbymethylthiazolyltetrazolium(MTT)method．Theapoptosisratesweremeasuredbyflowcytometry．Reactiveoxygenspecies(ROS)，02’。andmitochondrialmembranepo

3、tential(MMV)weredetectedusing2’，7'-dichlorodihy-drofl-uorescindiacetate(DCFH-DA)，dihydroethidium(DEH)andJC一1fluorescentprobes，respectively．Methanedicarboxylicaldehyde(MDA)，Superoxidedismutase(SOD)andATPweremeasuredbyMicroplateReadercorrespondingkits．TheconcentrationofCa2+andtheactivityofmembranem

4、itochondriltransitionpore(MPTP)weremeasuredbyfluorescencespectrophotometerusingFlo-3Mprobeandcalcein—AM-cobaltprobe．respectively．Caspase一3andcaspase-9activitiesweremeasuredbycorrespondingkits．TheproteinexpressionlevelsofcytochromeC(CytC)，B—celllymphoma02(Bcl一2)andBcl-2AssaciatedXprotein(Bax)werem

5、easuredbywesternblot．ThemRNAexpressionlevelsofCOQ2andCOQ4weremeasuredbyreal-timefluorescentquantitativePCR．Results：1．ToassaythechangesincellviabilityafterexposedtoCr(VI)，weevaluateditsdoseeffectsonculturedL一02hepatocyteswithorwithoutCoQ10pretreatment．WiththeincreasingconcentrationsofCr(VI)ranging

6、from0to8州，thecellviabilitywassignificantlydecreasedIV万方数据硕士学位论文英文摘要Q7

7、sofCoQl0onprogrammedcellulardeathafterCr(VI)exposure，weanalyzedcellapoptosisusingannexinV-FITCandpropidiumiodide(PI)doublestainingmethodsafterincubationfor30rain．Wit}ltheincreasingconcentrationsofCr(VI)rangingfrom1to4}