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ID:32916883
大小:2.84 MB
页数:72页
时间:2019-02-17
《高表达野生型或激活突变shp-2酪氨酸磷酸酶对乳腺癌细胞恶性生物学行为的影响及其机制的探讨》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、TABLEOFCONTENTSAbbreviations.....................................................................................................1Abstract(InChinese).........................................................................................4Abstract(InEnglish)............
2、..............................................................................7Introduction.......................................................................................................14Results...................................................................
3、...........................................44PartI.ConstructionofSHP-2EukaryoticExpressionVectorPartII.1EffectsofOverexpressionwildtypeorGain-of-FunctionMutationSHP-2TyrosinePhosphataseonMalignantBiologicalBehaviorsofBreasttumorcellsPartII.2TumorigensisinnudemiceDiscuss
4、ion........................................................................................................47Summary..........................................................................................................48References....................................
5、....................................................................56Appendix..........................................................................................................57Acknowledgement.....................................................................
6、......................58Review.............................................................................................................68AbbreviationsTBSTrisbufferedsalineSDS-PAGESDSpolyacrylamidegelelectrophoresisddH2Odi-distilledwaterECLenhancedchemiluminescencecD
7、NAcomplementaryDNAEBethidiumbromideDEPCdiethylpyrocarbonateFNFibronectinPCRPolymerasechainreactionBSAbovineserumalbuminEDTAethylenediaminetetraaceticacidSDSsodiumdodecylsulphatePMSFphenylmethylsulfonylfluorideEGFepidermalgrowthfactorODopticaldensityDMEMDulbecco’smodifie
8、dEagle’smediumFBSfetalbovineserumPBSphosphate-bufferedsalineErkextracellularsignal-regulatedkinasePI3Kphosphat
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