猴腺病毒检测方法的建立与初步应用

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1、中国食品药品检定研究院硕士研究生学位论文猴腺病毒检测方法的建立与初步应用姓名：张荣建学号：845030907790413专业：免疫学研究方向：实验动物病毒学导师姓名：贺争鸣研究员二零一二年七月目录一、中文摘要…………………………………………………………………………1二、英文摘要··················································································3三、引言···········································

2、·············································6四、第一部分猴腺病毒PCR检测方法的建立·······································8（一）实验材料··········································································8（二）实验方法········································································11

3、（三）实验结果········································································18（四）分析与讨论·····································································29（五）小结··············································································31五、第二部分G亚属猴腺病毒探针法荧光定量PCR检

4、测方法的建立········32（一）实验材料········································································32（二）实验方法········································································35（三）实验结果········································································40（四）分析与讨论··

5、···································································46（五）小结··············································································48六、第三部分猴腺病毒Ⅰ型血清学检测方法的建立······························49（一）实验材料······················································

6、··················49（二）实验方法········································································54（三）实验结果········································································64（四）分析与讨论·····································································79（五）小结···

7、···········································································81七、第四部分猴腺病毒GD株的分离与鉴定·······································82（一）实验材料········································································82（二）实验方法············································

8、····························84（三）实验结果········································································86（四）分析与