耳蜗雪旺细胞来源的wnt1对耳蜗移植干细胞分化的影响及其分子机制研究

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1、第四军医大学博士学位论文耳蜗雪旺细胞来源的Wnt1对耳蜗移植干细胞分化的影响及其分子机制研究专业名称：耳鼻咽喉科学　作者姓名：何亚　　　　　指导老师：邱建华　　　　论文编号：博士学位论文耳蜗雪旺细胞来源的Wnt1对耳蜗移植干细胞分化的影响及其分子机制研究培养类别学历研究生申请学位类型学术学位学科(领域)专业耳鼻咽喉科学研究方向耳聋的基础与临床二O一二年五月目录缩略语表·································································································

2、····················1中文摘要·····················································································································4英文摘要·····················································································································6前言···············

3、······································································································8文献回顾·····················································································································9正文·······················································

4、···························································18实验一螺旋神经元损伤动物模型的建立、鉴定···········································181材料···········································································································181.1实验动物·····································

5、························································181.2主要试剂·····························································································181.3主要仪器·····························································································192方法·················

6、··························································································192.1动物分组·····························································································192.2ouabain局部给药建立螺旋神经元损伤动物模型····························192.3听性脑干反应（auditorybrainstemresp

7、onse，ABR）检测···········202.4基底膜铺片·························································································202.5毛细胞计数·························································································212.6免疫荧光染色、HE染色··············································

8、····················212.7统