issr分子生物技术对中药蒲公英的应用研究

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1、河南中医学院２０１２届硕士研究生学位论文ISSR分子生物技术对中药蒲公英的应用研究研究生姓名：邱天宝导师：李喜凤教授陈随清教授指导组成员：张红梅副教授学科、专业：药物分析学所属院、系：药学院中国﹒郑州２０１２年４月３０日硕士学位论文原创性声明本人所呈交的硕士学位论文，是在导师李喜凤教授的指导下，独立进行科学研究工作所取得的成果。除文中已特别加以注明引用的内容外，论文中不包含任何其他个人或集体已经发表或撰写过的研究成果。对本文的研究做出重要贡献的个人和集体，均已在文中以明确方式标明并致谢。本人完全

2、意识到本声明的法律结果由本人承担。特此声明。论文作者（签名）：年月日硕士学位论文使用授权声明本人已完全了解河南中医学院有关保留、使用硕士学位论文的相关规定，同意学校保留或向国家有关部门、机构送交本学位论文的复印件和电子版，允许论文被查阅或借阅。本人授权河南中医学院可以将本论文的全部或部分内容编入有关数据库进行检索，可以采用影印、缩印或其他复制手段保存、汇编本论文（注：保密论文在解密后适用本授权声明）。特此声明。论文作者（签名）：导师（签名）：年月日年月日目录摘要·················

3、····················································································1Abstract····································································································3前言······················································

4、··················································5实验1蒲公英ISSR-PCR反应体系及扩增程序的建立与优化·······························7材料与方法·······························································································71试验材料································

5、·····························································72试验方法·····························································································82.1蒲公英基因组总DNA的提取·································································82.2正交试验

6、设计·····················································································82.3ISSR-PCR扩增···················································································82.4最佳退火温度的确定·····················································

7、·······················92.5ISSR-PCR扩增程序优化······································································92.6最佳方法的验证··················································································9结果与分析········································

8、·······················································9实验2蒲公英ISSR-PCR引物的筛选····························································13材料与方法······························································································131试验材料·······