蛋白质组学筛选肝癌差异蛋白及其与肿瘤分子成像特征的相关研究

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1、博士学位论文英文摘要m印sofhepatocellularcarcinomatissuesandthenormalhepatocellulartissueswereobtained，andmostoftheprotein—spotsdistributeintheareaof20"-"100KdaandpH4--一8．Thirteenproteindotswhichwerehigherexpressioninhepatocellularcarcinomagroupincludingwithorwithoutportalveinmetastasisthannormalgroupweres

2、electedformassspectrum．Thirteenproteinswereidentified，sevenproteinincludingannexinA3、laminBl、annexinA1、Transgelin．2、TCPl、PRP2、RANBPwereexpressedathigherlevelsinthegroupwithportalveinmetastasisthanthegroupwithoutportalveinmetastasis．LaminBl、annexinA3、HSP27．thethreeprotein’Sexpresslevelwereobserv

3、edbvW．estemBlotinhepatocellularcarcinomaandnormalhepatocellulartissue．Thethreeproteinsinhepatocellularcarcinomawerehigherexpressionthaninnormalhepatocellulartissue．Ⅵmilenm23一H1、Prohibitin、Triosephosphateisomerase(EC5．3．1．1)(TIM)threeproteinsinhepatocellularcarcinomawereexpressedatlowerlevelstha

4、ninnormalhepatocellulartissue．Weobservedtheexpression1evelofannexinA3andnm23．H1innormalhepatocellulartissueandhepatocellularcarcinomabyimmunohistochemicalmethod．TheannexinA3proteinexpressmasculinerateinnormalhepatocellulartissue，hepatocellularcarcinomawithoutportalveinmetastasisandhepatocellula

5、rcarcinomawithportalveinmetastasisiS10％．35％and92．5％．Varianceanalysisoftheresulthintmulti-groupcomparisonhavesignificantdifference(Z2=57．667，P<0．001)．‘阮furthercarriedouttwogroupcomparisonandshowsignificantdifferencesingroupsforannexinA3expressionamongnormalhepatocellulartissue，hepatocellularcarc

6、inomawithoutportalveinmetastasisandhepatocellularcarcinomawithportalveinmetastasis．Thenm23．H1proteinexpressedmasculinerateinnormalhepatocellulartissue．hepatocellularcarcinomawithoutportalveinmetastasisandhepatocellularcarcinomawithportalveinmetastasisis85％．82．5andl5％．Varianceanalysisoftheresult

7、showmulti-groupcomparisonhavesignificantdifference(X2=52．95，P<0．001)．Wefurthercarriedouttwogroupcomparisonanddiscoveredtheexpressionofnm23．H1iSnosignificantdif俺rencebetweennormalhepatocellulartissueandhepatocellularVcarCinomawitho