串珠素对成纤维细胞增殖影响及其和碱性成纤维细胞生长因子(bfgf)关系

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1、TheeffectofpelercanonproliferationoffibroblastanditsrelationsmpwithBFGFAbstractObjectiveToabservetheeffectofpelercanonproliferationoffibroblastanditsrelationshipwithBFGEMethodsEstablishmentoftheratlaminectomymodelonL4—5，takethescarconnectivetissueafter4weeks，primarycultureandpassageof,th

2、eratfibroblastswererandomlydividedintothreegroups：non—transfectedgroup，transfectedcontrolgroupandtransfectedgroup，FluorescencequantitativePCRdetecttheexpressionofmRNAofperlecan．WestemblottingdetectthequantityofperlecanandbFGF．MTTdetectthecellproliferationstatusofjoiningbFGFornot．Resultss

3、lowvirussh—perlecanweresuccessfullytransferredintoratfibroblasts，PerlecaninmRNAandproteinlevelsandbFGFwaslowexpressioninthetransfectedgroupandhighexpressionintheuntransfectedgroupandtransfectedcontrolgroup，withsignificantdifferencesrespectivelybetweenthetransfectedgroupandtheother2groups

4、(P<0．05)，Incultureof0．1％FBS1640medium，thecellproliferationratewasdecreasedinuntransfectedgroup，transfectedcontrolgroupandtransfectedgroup，butcomparedtotheuntransfectedgroupandtransfectedcontrolgroup，thecellproliferationrateWassignificantlylowerintransfectedgroup．Incultureof0．1％FBSjoining

5、inbFGFoflgg／1，fibroblastcellproliferationWashardlynormalintheuntransfectedgroupandtransfectedcontrolgroup，buttheabilityofcellproliferationwaslowerintransfectedgrouD．ConclusionTransfectionofratfibroblast、析thSlowvirusshPerlecandown·regulatedtheexpressionofperlecan，Cellproliferationratedecl

6、ined，theexpresssionofbFGFreduced，thereactionofratsfibroblaststobFGFreducedintransfectedgroup．Postgraduate：liuweiliang(Spinesurgery)DirectbyProf：ZouyunwenKeywords：Fibroblasts；Perlecan；Lowvirus；Transfection；bFGF目录引言⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯1实验试剂与方法⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯21．1实验试剂⋯⋯

7、⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．．21．2实验分组与方法⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯21．3统计学方法⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯9第2章结果⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯102．1PCR结果⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯102．2WesternBlot结果⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯102．3MTT结果⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯11第3章讨论⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯