不同部位肋软骨ⅱ型胶原蛋白差异性比较

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1、AbstractComparisonoftypeIIcollagenindifferentpartsofcostalcartilageObjective：BycomparingtheamountoftypeIIcollagenamongtheautologous6th，7th，and8thcostalcartilages，toprovidetheoreticalbasisofautologouscartilagetransplantationatthemolecularlevel．Methods：From8to12yearsoldpatientsperformedearreconst

2、ructionsu唱erywithautologouscostalcartilage，collectingevery20costalcartilagetissuesamplesof6mrib，7mrib．and8mrib．Dividingintothreegroupsaccordingbydifferentsourcesofparts．Eachgroupcontents20samples，and60samplestotal．ThelevelofmRNAoftypeIIcollageninthe6m，7m，8‘“costalcartilagesinpatientsweretestedb

3、yflorescentreal—timequantitativePCR．TheproteincontentoftypeIIcollagenandtheamountofchondrocytesintheautologous6th，7th，8thcostalcartilageinpatientsweredetectedbyimmunohistochemistryandHEstaining．Results：1)thereisnosignificantlydifferenceoftheleveloftypeIIcollagenmRNAamong6th，7th，and8thcostalcart

4、ilages(9．3士1．2，8．984-0．5，9．36±1．3)；2)thereisnosignificantlydifferenceoftherelativegrayvalueoftypeIIcollagenin6th，7th，and8thcostalcartilages(14517．84-398，15368．3士736，13992．05±802)；3)thereisnosignificantlydifferenceoftheamountofchondrocytesin6th，7th，and8thcostalcartilages．Conclusion：Throughthisst

5、udyfoundoutthatthereisnosignificantdifferenceinthecomparisonofmRNAexpressionandrelativegrayvaluesintypeIIcollagenin6搬．7mand8mrib．Also，thereisnosignificantdifferenceinthecomparisonofcostalcartilagecellcountin6th，7tnand8thrib．Thisresultcanbeconcludedthatduringautologouscartilagetransplantation，wh

6、ichconsideratedofselectingdawningautologouscartilagetissuepartsatthemolecularlevel，isnosignificantdifferencein6m．7‘“or8mrib．。Postgraduatestudent：Zhong—HangZhang(Plasticsurgery)DirectedbyAssociate：prof．Zhen—YuChenKeywords：typeIIcollagen；costalcartilage；autologouscartilagetransplantation目录亩ff言⋯．⋯

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