肝癌靶向sea-cd80共表达重组腺病毒的构建

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1、GeneratedbyFoxitPDFCreator©FoxitSoftwarehttp://www.foxitsoftware.comForevaluationonly.第四军医大学硕士学位论文独创性声明秉承学校严谨的学风与优良的科学道德，本人声明所呈交的论文是我个人在导师指导下进行的研究工作及取得的研究成果。尽我所知，除了文中特别加以标注和致谢的地方外，论文中不包含其他人已经发表或撰写过的研究成果，不包含本人或他人已申请学位或其他用途使用过的成果。与我一同工作的同志对本研究所做的任何贡献均已在论

2、文中作了明确的说明并表示了致谢。申请学位论文与资料若有不实之处，本人承担一切相关责任。论文作者签名：日期：保护知识产权声明本人完全了解第四军医大学有关保护知识产权的规定，即：研究生在校攻读学位期间论文工作的知识产权单位属第四军医大学。本人保证毕业离校后，发表论文或使用论文工作成果时署名单位仍然为第四军医大学。学校可以公布论文的全部或部分内容（含电子版，保密内容除外），可以采用影印，缩印或其他复制手段保存论文。学校有权允许论文被查阅和借阅，并在校园网上提供论文内容的浏览和下载服务。同意学校将论文加入《

3、中国优秀博硕士学位论文全文数据库》和编入《中国知识资源总库》，同意按《中国优秀博硕士学位论文全文数据库出版章程》规定享受相关权益。论文作者签名：导师签名：日期：2GeneratedbyFoxitPDFCreator©FoxitSoftwarehttp://www.foxitsoftware.comForevaluationonly.第四军医大学硕士学位论文肝癌靶向SEA-CD80共表达重组腺病毒的构建研究生：温银田学科专业：老年医学所在单位：第四军医大学西京医院老年病科导师：张丙芳教授辅导教师：叶菁

4、副教授资助基金项目：国家“重大新药创制”科技重大专项（2009ZX09102）关键词：原发性肝癌；免疫基因治疗；腺病毒；甲胎蛋白；葡萄球菌肠毒素A；CD80中国人民解放军第四军医大学2010年5月3GeneratedbyFoxitPDFCreator©FoxitSoftwarehttp://www.foxitsoftware.comForevaluationonly.第四军医大学硕士学位论文目录缩略语表·················································

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7、·······················································14文献回顾····························································································································································16实验一携带人AFP启动子和增强子序列的腺病毒穿梭质粒的构建····

8、···························250.引言···························································································································251.实验材料········································································