运动心脏重塑过程中大鼠左室肌蛋白质组差异表达的研究

《运动心脏重塑过程中大鼠左室肌蛋白质组差异表达的研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、upregulatedafterexercise，ATPsynthasecouplingfactor6(CF6)andanunknownproteinwhoseMris116kDaaredownregulatedafterexercise．Conclusions：①Theratheartoftheexercisegroupsincreasedafter12weeks’mediumintensitytraining．(室)Duringtheremodelingprogressofthetestgroupshe

2、art，theproteomeoftheleftventricularmusclehasanotabledifferentialexpressionchangeafter4weeks’training，andtheproteomehasanadaptivechangesafter8，12weeks’training．③Usingthemediumintensitytrainingtohaveaheartremodeling，thetrainingratheartshaveawelladaptivechang

3、es．thiscanimprovethelevelofcardiacmuscleenergymetabolism，enstrengthentheabilityofcardiacmusclestressreactionandadaption，andmelioratethebloodpressureadjustmentabilities．Desucceedinfiltrating4objectiveproteinswhicharenotmentionedinthesportsmedicineareaatpres

4、ent，theyare78kDaglucose—regulatedprotein，Enoyl—CoAhydratase，ATPsynthasesubunitalphaandATPsynthasecouplingfactor6，etc．thisworkestablishesaprophaseworkbasictohaveafurtherstudyontheathleticheart．KeyWord：athleticheart：leftventricularmuscle；mediumintensitytrain

5、ing：proteome：differentialexpression．VACN缩略词表(中英文对照)乙腈(Acetonitrile)Acr丙烯酰胺(acrylamide)APS过硫酸铵(ammoniumpersulfate)Bis甲叉丙烯酰胺【N，N'-Methylene—bis一(arylamide)】BSA牛血清白蛋白(BovineSerumAlbumin)CHAPS3．【(3-胆酰胺丙基)-乙二胺】-1-丙磺酸ddH20双蒸水(doubledistilledwater)2-DE双向凝胶电泳(two-

6、dimensionalgelelectrophoresis)D'IT二硫苏糖醇(dithiothreit01)EDTA乙二胺四乙酸(enthylenediaminetetra—aceticacid)IAA碘乙酰胺(iodoacetamide)IEF等电聚焦(isoelectricfocusing)IPG固相PH梯度(immobilizedPHgradient)Mr分子量(molecularweight)NP．40非离子去垢剂(NonidetP40)PAGE聚丙烯酰胺凝胶电泳(polyacrylamidege

7、lelectrophoresis)PMF肽指纹图谱(peptidemassfingerprint)PMSF蛋白酶抑制剂(phenylemthansulfonylfluoride)pI等电点(isoelectricpoint)SDS十二烷基硫酸钠(sodiumdodecylsulfate)SSP标准点编号(standardspotnumber)TEMED四甲基乙二胺(N，N，N’，N；．tetramethyl—ethlenediamine)Tris三羟甲基氨基甲烷(tfis一(hydroxymethyl)am

8、inomethane)MALDI．TOF-TOF．MS基质辅助激光解析电离飞行时间串联质谱(Matrix—assistedlasertwo·stagedesorption／ionizationtime-of-flighttandemmassspectrometry)55湖南师范大学学位论文原创·l生声明本人郑重声明：所呈交的学位论文，是本人在导师的指导下，独立进行研究工作所取得的成果。除文中已经注明引用的内容外，