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ID:32605033
大小:4.76 MB
页数:75页
时间:2019-02-13
《补阳还五汤促进周围神经损伤修复的理论和实验研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、experimentwasdesignedtostudytheeffectofBuyangHuanwuDecoctionontheperipheralnerveregeneration.METHODS:Twoexperimentswereprocessedinfore-and-afttime.Inthefirstexperiment,Spragus—Dawelyratswereusedandrandomlydividedintothreegroups:BuyangHuanwuDecoctiongroup,Methycobalgroupandcontrolgroupafterthes
2、ciaticnerveWascrushed.Onedaypost—operatively,ratswerefeddistilledwater'Methycobal(62.5ug/1009)andBuyangHuanwuDecoction(1.309/1009)respectively.Eachgroupmedicinewasdiluted2mlbydistilledwater.Theratsallweredosed2mleveryday.Inthesecondexperiment,equalfemaleandmaleSpragus·Dawelyratswereusedandrand
3、omlydividedintofivegroups:threeBuyangHuanwuDecoctiongroups(10w,middleandhighdose),MethycobalgroupandcontrolgroupafterthecommonperonealnerveWascrushed.ThedoseofBuyangHuanwuDecoctionWasdifferent,whichWas1.309/1009,0.659/1009andO.33g/lOOgrespectively.EachgroupmedicineWasdiluted4mlbydistilledwater
4、.Theratsallweredosed2mleachtimeand2timeseachday.Regularobservationandevaluationofthelocomotionofinjuredlimbswasconducted.After6daysoftheoperation,sciaticfunctionindex(SFI)ortoe—spreadfactorindex(TEl)wastestedevery3days.Theexperimentalindexesweredetectedontherighttime,whenthedifferenceisstatist
5、icaldistinct.Nerveeletrophysiologicalexaminationandimmunohistochemistrywereperformedtoassessthenerveregenerationoutcome.Inaddition,histologicalassessment,includinglightmicroscopy,transmissionelectronmicroscopyandmorphometricanalyseswereappliedtobothregeneratednervesandtargetmusclestoevaluateth
6、eeffectsofnerveregeneration.RESULTS:TheresultsshowthatalltheindexesinBuyangHuanwuDecoctiongroupsweresignificantlypriortothecontrolgroup.Forexample,comparingwiththecontrolgroup,thehigherrecoveryofSFIandTFI,thebetterformofgastrocnemiusandaxon.Thenervalelectrophysiologydetectionshowthat,theaverag
7、eproximalCMAPamplitudewas:thehighdoseBuyangHuanwuDecoctiongroup:4.46mv;themiddledoseBuyangHuanwuDecoctiongroup:3.36mv;thelowdoseBuyangHuanwuDecoctiongroup:2.91mv;theMethycobalgroup:2.78mv;thecontrolgroup:2.63mv.ThehighdoseBuyangHuanwuDe
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