电动流动全分析系统研究及其在农药残留分析中的应用

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1、Abstractmechanicalstrengthofthesilicagelwasstrongenoughandthesilicagelsolidphasewasabletobeadoptedinorganicsolvents．Octylfunctiongroupdistributedinthecolumn，resultinginfleeofchemicallymodifiedproceduceforstationaryphaseandimprovementofreproducibility．Thescanningelectromicrographdispla

2、yedthatthemicrocolumnpossessedhighpermeabilityandcouldbeemployedforchromatographicseparation．2．AtechniqueforpreparationofCs-quartzsandmonolithicmicrocolumnbyone—steppolycondensationwasproposed．Theheatingeffectincreasedacutelywiththeaccretionofdiameterofseparationcolumndiametertomillim

3、eterdimension，whichresultedinseriousdiffusenessofseparationzone．BasedonthepreparationofC8一silicamonolithiccolumnmentioned，10cmlengthand2．0mmi．d．electrochromatographicmicrocolumnfilledwith120—150orderquartzsandWasprepared，whichCallbenamedasquaazsandmonolithicmicrocolumn,whichwasusedtoi

4、mprovethesampleloadabilityandthedetectionsensitivity．Theheatingeffectwasalsorestrictedbypackingfinequartzsandintothesilicagel，becausetheelectriccurrentinthequartzsandmonolithicmicrocolumnWasmuchlowerthanthatofthenormalmonolithicmicrocolumnunderthesameelectricfieldstrength．Ontheotherha

5、nd，theshrinkageassociatedwiththepolymerizationWaseliminatedpracticallybypackingfinequartzsandintothefusedsilicatube，andthemicrocolumnandthewalloffusedsilicatubecouldbecontactedtightly．BasedonthetransportationoftheEFAandseparationofEC，aseparationsystemwiththemicrocolumnandon—lineUVdete

6、ctionwasassembled．Theanalyticalsystemwasofsafety,convenienceandautomatization．Electroosmoticflow(EOF)orEOFcombinedwithpressureflowwasusedtodrivemobilephaseinECofthemicrocolumn．AroutinespectrophotometerwasusedtodeterminetheanalytesseparatedbytheECminrocolurnn。Separationparametersthataf

7、fectedthecolumnefficiencywereexplored，suchasconcentrationoforganicsolventinmobilephase，bufferpH，electricfieldstrengthandflowrateofelctroosmoticpump．Thecolumnefficiencycouldachieve6052plates／mundertheconditionasfollowing：65％acetonitrile(v／v)containinglmmol／LTrisbuffersolution(pH8．0)asm

8、obile